目的 建立淫羊藿Epimedium brevicornu中化學(xué)成分的超高壓液相色譜–飛行時(shí)間質(zhì)譜（UPLC/Q-TOF-MS）的分析方法。方法 Acquity UPLC BEH C18色譜柱（100 mm×2.1 mm，1.7 μm），流動(dòng)相為0.1%甲酸水溶液–乙腈，梯度洗脫；體積流量為0.4 mL/min；柱溫為45 ℃；進(jìn)樣量為2 μL。質(zhì)譜檢測(cè)采用正、負(fù)離子模式，電壓分別為3.0、2.5 kV；離子源溫度110 ℃；霧化溫度400 ℃；霧化氣體積流量800 L/h。結(jié)果 在10 min內(nèi)完成淫羊藿中40個(gè)化學(xué)成分的鑒定。結(jié)論 建立了一種簡單、快速、高效的UPLC/Q-TOF-MS方法對(duì)淫羊藿中化學(xué)成分進(jìn)行了鑒定，為全面控制淫羊藿的質(zhì)量和機(jī)理研究提供了基礎(chǔ)。

Objective To establish a method for the analysis on the chemical constituents in Epimedii Herba by UPLC/Q-TOF-MS. Methods The method was performed on Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm), and the mobile phase consisted of 0.1% formic acid aqueous solution and acetonitrile with gradient elution program. The injection volume was 2 μL at the flow rate of 0.4 mL/min. The column temperature was 45 ℃. Both positive and negative modes were selected for qualitative analysis. The capillary voltages were set at 3.0 and 2.5 kV. The ion source temperature and atomization temperature were 110 and 400 ℃. The flow rate of nebulization gas was 800 L/h. Results The main peaks were separated clearly and respectively in 10 min, and 40 compounds were identified. Conclusion The established method for the determination of the chemical constituents in E. brevicornu using UPLC/Q-TOF-MS is simple, rapid, and reliable, and paves a way for the quality control and further study of Epimedii Herba.