目的 建立同時(shí)測(cè)定銀屑優(yōu)化顆粒中芍藥苷、甘草苷、落新婦苷、迷迭香酸和甘草酸5種成分的HPLC-DAD測(cè)定方法。方法 采用高效液相色譜波長(zhǎng)切換法。Lichrospher C18色譜柱（250 mm×4.6 mm，5 μm）；流動(dòng)相為乙腈–0.1%磷酸溶液，梯度洗脫；檢測(cè)波長(zhǎng)在230、254、290 nm切換；體積流量為1 mL/min；柱溫為30 ℃；進(jìn)樣體積10 μL。結(jié)果 5種成分的線性關(guān)系良好。芍藥苷在69.12～691.20 ng線性關(guān)系良好，平均加樣回收率為97.45%，RSD值為1.54%；甘草苷在39.02～390.20 ng線性關(guān)系良好，平均加樣回收率為97.10%，RSD值為1.90%；落新婦苷在30.03～300.30 ng線性關(guān)系良好，平均加樣回收率為99.98%，RSD值為1.22%；迷迭香酸在7.82～78.20 ng線性關(guān)系良好，平均加樣回收率為100.83%，RSD值為1.36%；甘草酸在31.09～310.90 ng線性關(guān)系良好，平均加樣回收率為97.52%，RSD值為1.49%。結(jié)論 本方法具有方法簡(jiǎn)便、快速、準(zhǔn)確等特點(diǎn)，可同時(shí)測(cè)定銀屑優(yōu)化顆粒中5種活性成分。

Objective To establish a quantification method to determine paeoniflorin, liquiritin, astiblin, rosmarinic acid, and glycyrrhizic acid in Yinxie Youhua Granules by HPLC-DAD method. Methods The components were determined by HPLC simultaneously with changing ultraviolet-visible wavelength. The column was Lichrospher C18 (250 mm×4.6 mm，5 μm). The mobile phase was acetonitrile- 0.1% phosphoric acid solution in gradient elution mode. The detection wavelength was set at 230, 254 and 290 nm. The injection volume was 10 μL at the flow rate of 1.0 mL/min. The temperature of column was set at 30 ℃. Results The calibration curves of five active components were excellent. Paeoniflorin had a good linearity in the range of 69.12 — 691.20 μg and its average recovery was 97.45% with RSD of 1.54%. Liquiritin had a good linearity in the range of 39.02 — 390.20 μg and its average recovery was 97.10% with RSD of 1.90%. Astiblin had a good linearity in the range of 30.03 — 300.30 μg and its average recovery was 99.98% with RSD of 1.22%. Rosmarinic acid had a good linearity in the range of 7.82 — 78.20 μg and its average recovery was 100.83% with RSD of 1.36%. Glycyrrhizic acid had a good linearity in the range of 31.09 — 310.90 μg and its average recovery was 97.52% with RSD of 1.49%. Conclusion The established method is convenient and accurate with a satisfactory separation and high sensitivity. It can be used to determine the five components in Yinxie Youhua Granules with the same chromatogram condition.