目的 利用HPLC-DAD法建立苦碟子中木犀草素7-O-β-D-葡萄糖苷（LGCOP）、木犀草素7-O-β-D-葡萄糖醛酸苷（LGCRP）和芹菜素7-O-β-D-葡萄糖醛酸苷（AGCRP）的測定方法。方法 Diamonsil C18色譜柱（200 mm×4.6 mm，5 μm）；流動相為（0.05%甲酸–水）–（0.05%甲酸–乙腈）；二元梯度洗脫：檢測波長340 nm；體積流量1.0 mL/min；柱溫35 ℃；進(jìn)樣量10 μL。結(jié)果 在選定色譜條件下線性關(guān)系良好（r≥0.999 9）。平均回收率分別為100.5%、100.1%、100.8%，RSD值分別為0.4%、0.3%、0.7%。結(jié)論 該分析方法能簡便、快速地測定苦碟子中黃酮類成分，可為評價不同產(chǎn)地、藥用部位及采收期的藥材提供科學(xué)依據(jù)。

Objective To establish an analysis method for simultaneous determination of luteolin-7-O-β-D-glucopyranoside (LGCOP), luteolin-7-O-β-D-glucuronopyranoside (LGCRP) and apigenin-7-O-β-D-glucuronopyranoside (AGCRP) in Ixeris sonchifolia by HPLC-DAD. Methods HPLC was carried out on a Diamonsil C18 ( 200 mm× 4.6 mm, 5 μm) column with (0.05% formic acid- water) - (0.05% formic acid -acetonitrile) as mobile phase for two elements of gradient elution. The detection wavelength was set at 340 nm. The injection volume was 10 μL at the flow rate of 1.0 mL/min. The temperature of column was set at 35 ℃. Results There were good linear relationship of LGCOP, LGCRP, and AGCRP (r≥0.9999). The average recoveries were 100.5%, 100.1%, and 100.8% with RSD values of 0.4%, 0.3%, and 0.7%, respectively. Conclusion The method is convenient, specific, and can be used for the quality control of flavonoids in I. sonchifolia from different areas, various medicinal parts, and different harvest.

國家科技重大專項（2011ZX09401-305-42）；國家自然科學(xué)基金青年基金項目（81102732）