-1·d-1)、rhGH(0.25 mg·kg-1·d-1)、PEG-rhGH(1.4 mg·kg-1·周-1)處理。4周后進(jìn)行糖耐量實(shí)驗(yàn)和胰島素釋放試驗(yàn),計(jì)算胰島素曲線面積與葡萄糖曲線面積比值,胰島素穩(wěn)態(tài)模型(HOMA-IR);檢測(cè)血清生長(zhǎng)抑素水平;免疫組織化學(xué)法檢測(cè)胰島胰島素(INS)、胰高血糖素(GLU)、生長(zhǎng)抑素(SS)、胰多肽(PP)。結(jié)果 PEG-rhGH組HOMA-IR較對(duì)照組、模型組低(P<0.05),與rhGH組比較無差異。AUCI/AUCG值組間比較顯示PEG-rhGH組高于rhGH組,但無統(tǒng)計(jì)學(xué)差異。PEG-rhGH組GLU蛋白表達(dá)與模型組表達(dá)無差異。PEG-rhGH組INS表達(dá)較模型組表達(dá)上升(P<0.05)。PEG-rhGH組SS、PP表達(dá)與rhGH無差異。血清SS各組間均無明顯差異。結(jié)論 未觀察到PEG-rhGH引起去垂體大鼠胰島素抵抗和胰島β細(xì)胞分泌能力下降,對(duì)胰島分泌蛋白的表達(dá)無明顯影響。;Objective To observe whether the long-acting growth hormone polyethylene glycol recombinant human growth hormone (PEG-rhGH) causing insulin resistance or not, and the differences with the short-acting growth hormone recombinant human growth hormone (rhGH). Methods Sprague-Dawley young rats (106 rats) weighing 60 — 80 g were underwent hypophysectomy via parapharyngeal approach, and 18 rats with Sham operation were selected into the control group. Fifty-four qualified rats were randomly divided into the model, rhGH, and PEG-rhGH groups after two weeks, which were administered with saline (0.25 mg·kg-1·d-1), rhGH (0.25 mg·kg-1·d-1), and PEG-rhGH (1.4 mg·kg-1·week-1), respectively. After treatment for 4 weeks, glucose tolerance test and insulin release test were performed to calculate area under the curve insulin (AUCI), area under the curve glucose (AUCG), and homeostasis model assessment (HOMA-IR). Serum somatostatin (SS) levels were determined. Immunohistochemistry displayed the insulin (INS), glucagon (GLU), SS, and pancreatic polypeptide (PP). Results Glucose tolerance test and insulin release test indicated that HOMA-IR in PEG-rhGH group declined compared with those in the control and model groups (P< 0.05), and there were no difference between rhGH and PEG-rhGH groups. The value of AUCI/AUCG in PEG-rhGH group was higher than that of rhGH group without significant difference. Protein expression of GLU indicated no difference among PEG-rhGH, control, and model groups. The INS expression in PEG-rhGH group increased compared with the control group (P<0.05). SS and PP expression in PEG-rhGH group had no difference compared with that in rhGH group, and there was no significant difference of SS in serum among each group. Conclusion Insulin resistance and decreased islet β cell secretory capacity are not observed, and no significant effects of PEG-rhGH on secretion function of pancreas islet in hypophysectomized rats are observed."/>

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首頁 > 過刊瀏覽>2014年第29卷第5期 >2014,29(5):465-470. DOI:10.7501/j.issn.1674-5515.2014.05.004
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聚乙二醇化重組人生長(zhǎng)激素對(duì)去垂體幼鼠胰島分泌的影響及其機(jī)制

Effect of pegylation recombinant human growth hormone on secretion function of pancreas islet in hypophysectomized rats and its mechanism

發(fā)布日期:2014-06-12