目的 建立以氫氧根作為淋洗液的離子色譜法測定復(fù)方氨基酸注射液（20AA）中醋酸根的方法。方法 使用Dionex ICS 3000離子譜儀，Dionex As19-Ag19陰離子交換色譜柱（250 mm×4 mm），水–30 mmol/L氫氧化鈉溶液為淋洗液，梯度洗脫，體積流量1.0 mL/min，柱溫30 ℃，進(jìn)樣量20 μL，抑制型電導(dǎo)檢測器，檢測池溫度35 ℃。結(jié)果 醋酸根與相鄰雜質(zhì)的分離度為1.8，最低檢出濃度0.75 μmol/L，線性范圍0.75～2.24 mmol/L（r=0.999 5），平均回收率為100.7%，RSD值為1.7%。結(jié)論 該方法操作簡單，分離度好，背景低，是控制復(fù)方氨基酸注射液（20AA）中醋酸根的可靠方法。

Objective To establish a method for the determination of acetate ion in Compound Amino Acid Injection (20AA) by ion chromatography with hydroxide as mobile phase. Methods Determination was carried out on Dionex As19-Ag19 anion-exchange chromatography column (250 mm × 4 mm) with Dionex ICS 3000 Ion Spectrometer. The elutriant consisted of water-30 mmol/L sodium hydroxide with gradient elution at a flow rate of 1.0 mL/min. The temperature of column was set at 30 ℃ with injection volume of 10 μL. Inhibitory electrical conductivity detector was used with detecting pool temperature of 35 ℃. Results The resolution between acetate ion peak and adjacent peak was 1.8, and LOD was 0.75 μmol/L. The calibration curve was linear in the range of 0.75 — 2.24 mmol/L (r = 0.999 5). The recovery was 100.7% with RSD value of 1.7% (n = 9). Conclusion The method is simple with good resolution and low background, and can be used for quality control of acetate ion in Compound Amino Acid Injection (20AA).