目的 建立測定六經(jīng)頭痛片中胡薄荷酮的高效液相色譜法。方法 采用Agilent Zorbax SB C₁₈色譜柱（250 mm×4.6 mm，5 μm）；以乙腈–0.05%磷酸水溶液為流動相，進行梯度洗脫；檢測波長256 nm；柱溫30 ℃；體積流量1.0 mL/min；進樣量10 μL。結果 胡薄荷酮在0.442 3～1 105.8 ng與峰面積線性良好（r=0.999 9），平均回收率為97.59%，RSD值為1.54%（n=6）。結論 建立的方法專屬性強、準確、快速，可有效地控制六經(jīng)頭痛片的質量。

Objective To establish an HPLC method for the determination of pulegone in Liujing Toutong Tablets. Methods The separation of pulegone was carried out by using Agilent Zorbax SB C₁₈ column (250 mm × 4.6 mm, 5 μm) with the mobile phase containing acetonitrile - 0.05% phosphoric acid aqueous with gradient elution. The detective wavelength was set at 256 nm. The flow rate was 1.0 mL/min with injection volume of 10 μL. Results The linearity range of pulegone was 0.442 3 — 1 105.8 ng (r=0.999 9), and the average recovery was 97.59% with RSD value of 1.54% (n=6). Conclusion The method is simple and accurate with good recovery and repeatability, and suitable for the quality control of Liujing Toutong Tablets.