18色譜柱(250 mm×4.6 mm,5 μm);以乙腈–0.05%磷酸水溶液為流動(dòng)相,進(jìn)行梯度洗脫;檢測(cè)波長(zhǎng)256 nm;柱溫30 ℃;體積流量1.0 mL/min;進(jìn)樣量10 μL。結(jié)果 胡薄荷酮在0.442 3~1 105.8 ng與峰面積線性良好(r=0.999 9),平均回收率為97.59%,RSD值為1.54%(n=6)。結(jié)論 建立的方法專屬性強(qiáng)、準(zhǔn)確、快速,可有效地控制六經(jīng)頭痛片的質(zhì)量。;Objective To establish an HPLC method for the determination of pulegone in Liujing Toutong Tablets. Methods The separation of pulegone was carried out by using Agilent Zorbax SB C18 column (250 mm × 4.6 mm, 5 μm) with the mobile phase containing acetonitrile - 0.05% phosphoric acid aqueous with gradient elution. The detective wavelength was set at 256 nm. The flow rate was 1.0 mL/min with injection volume of 10 μL. Results The linearity range of pulegone was 0.442 3 — 1 105.8 ng (r=0.999 9), and the average recovery was 97.59% with RSD value of 1.54% (n=6). Conclusion The method is simple and accurate with good recovery and repeatability, and suitable for the quality control of Liujing Toutong Tablets."/>