目的 建立高效液相色譜法測定大鼠血清中視黃醇和視黃酸濃度的方法。方法 色譜柱為Phenomenex Luna C₁₈柱（150 mm×4.6 mm，5 μm）；流動相為甲醇-10 mmol/L乙酸銨緩沖鹽（90：10，乙酸調整水相pH值至4）；體積流量為1.0 mL/min；檢測波長為340 nm；柱溫為40℃；進樣量為20 μL。結果 大鼠血清樣品中視黃醇和視黃酸均分離良好，視黃醇和視黃酸質量濃度在31.25～1 000 ng/mL（r=0.999 9）與峰面積均呈良好的線性關系。平均回收率大于90%，RSD值均小于10%。結論 本法操作簡便、準確、靈敏、專屬性強，為建立無干擾的測定大鼠血清中視黃醇和視黃酸的方法及其在大鼠體內藥動學研究提供參考。

Objective To establish an efficient and accurate method for detecting the content of retinol and retinoic acid in rat serum by HPLC. Methods The chromatographic separation was carried out on a Phenomenex C₁₈ column (150 mm × 4.6 mm, 5 μm) at a flow rate of 1.0 mL/min. Methanol-10 mmol/L ammonium acetate buffer solution (90:10, pH 4 adjusted with acetic acid) was used as mobile phase. It was monitored at a wavelength of 340 nm, the column temperature was 40℃, and the injection volume was 20 μL. Results The retinol and retinoic acid in rat serum also had a good linearity at 31.25-1 000 ng/mL (r=0.999 9). The average recoveries were above 90% with RSD values less than 10%. Conclusion This method is accurate, simple, and sensitive,which is suitable for the establishment of non-interference determination of retinol and retinoic acid in rat serum.

中華醫(yī)學會醫(yī)學教育研究課題資助項目（2012-SY-44）