目的 建立高效液相色譜法測(cè)定大鼠血清中視黃醇和視黃酸濃度的方法。方法 色譜柱為Phenomenex Luna C₁₈柱（150 mm×4.6 mm，5 μm）；流動(dòng)相為甲醇-10 mmol/L乙酸銨緩沖鹽（90：10，乙酸調(diào)整水相pH值至4）；體積流量為1.0 mL/min；檢測(cè)波長(zhǎng)為340 nm；柱溫為40℃；進(jìn)樣量為20 μL。結(jié)果 大鼠血清樣品中視黃醇和視黃酸均分離良好，視黃醇和視黃酸質(zhì)量濃度在31.25～1 000 ng/mL（r=0.999 9）與峰面積均呈良好的線性關(guān)系。平均回收率大于90%，RSD值均小于10%。結(jié)論 本法操作簡(jiǎn)便、準(zhǔn)確、靈敏、專屬性強(qiáng)，為建立無(wú)干擾的測(cè)定大鼠血清中視黃醇和視黃酸的方法及其在大鼠體內(nèi)藥動(dòng)學(xué)研究提供參考。

Objective To establish an efficient and accurate method for detecting the content of retinol and retinoic acid in rat serum by HPLC. Methods The chromatographic separation was carried out on a Phenomenex C₁₈ column (150 mm × 4.6 mm, 5 μm) at a flow rate of 1.0 mL/min. Methanol-10 mmol/L ammonium acetate buffer solution (90:10, pH 4 adjusted with acetic acid) was used as mobile phase. It was monitored at a wavelength of 340 nm, the column temperature was 40℃, and the injection volume was 20 μL. Results The retinol and retinoic acid in rat serum also had a good linearity at 31.25-1 000 ng/mL (r=0.999 9). The average recoveries were above 90% with RSD values less than 10%. Conclusion This method is accurate, simple, and sensitive,which is suitable for the establishment of non-interference determination of retinol and retinoic acid in rat serum.

中華醫(yī)學(xué)會(huì)醫(yī)學(xué)教育研究課題資助項(xiàng)目（2012-SY-44）