目的 觀察催產素對3T3-L1脂肪細胞糖脂代謝的影響.方法 3T3-L1前脂肪細胞體外培養(yǎng),并誘導其分化成熟為脂肪細胞.研究催產素對脂肪細胞葡萄糖消耗量以及三酰甘油、游離脂肪酸和甘油的影響.采用實時熒光定量PCR法檢測糖脂代謝相關基因GLUT-1、GLUT-4、ATGT、HSL的mRNA表達.結果 與對照組比較,催產素20、50、100 μg/mL組葡萄糖消耗量有所增加,且表現出劑量相關.催產素組較對照組的三酰甘油降低,而甘油和游離脂肪酸增高.催產素50 μg/mL組中脂代謝相關基因HSL表達明顯高于對照組,糖代謝相關基因GLUT-4 mRNA表達水平增加.結論 催產素處理可減少3T3-L1細胞脂質合成、增加脂質分解作用,并可明顯改善脂質積聚.

Objective To study the effect of oxytocin on glucose and lipid metabolism in 3T3-L1 adipocytes. Methods Preadipocytes from 3T3-L1 cell line were cultured in vitro and induced to differentiate to adipocytes. Mature adipocytes were treated with oxytocin. Glucose consumption, triglyceride, free fat acid, and glycerol levels were determined. The mRNA expression of differentiation marker genes such as GLUT-1, GLUT-4, ATGT, and HSL were evaluated by RT-PCR method. Results The glucose consumption in the oxytocin (20, 50, and 100 μg/mL) groups were increased with dose-dependent relationship compared with control group. Triglyceride in the oxytocin group was lower than that in control group, while glycerol and free fatty acid decreased. There was significant increase of expression levels of lipid metabolism related gene HSL and sugar metabolism related gene GLUT-4 mRNA in the oxytocin (50 μg/mL) group compared with control group. Conclusion Treatment of oxytocin may reduce 3T3-L1 cell lipid synthesis, increase lipid decomposition, and obviously improve lipid accumulation.