目的 優(yōu)化苦豆子多糖的脫色條件.方法 以多糖脫色率和多糖保留率為指標,考察雙氧水(H₂O₂)對苦豆子多糖溶液的脫色效果.通過單因素試驗考察H₂O₂脫色方法中雙氧水加入量、脫色時間、脫色溫度和pH值對苦豆子多糖脫色效果的影響,進一步采用正交試驗確定苦豆子多糖脫色的最佳條件.結果 H₂O₂對苦豆子多糖最佳脫色條件為脫色溫度60 ℃,調節(jié)pH 5,加入1.0%的H₂O₂,脫色反應3 h.在此條件下經驗證,多糖脫色率和多糖保留率可分別達到29.83%、71.12%.結論 H₂O₂脫色具有較好的脫色效果,可為生產出高品質的苦豆子多糖提供依據(jù).

Objective To optimize the decolorization conditions on Sophora alopecuroides polysaccharide. Methods The effects of H₂O₂ on decolorization of S. alopecuroides polysaccharide were studied with polysaccharide decolorization rate and polysaccharide retention rate as indexes. The amount of H₂O₂, bleaching time, bleaching temperature, and pH value were studied through single factor test in H₂O₂ bleaching method, and the further optimal conditions of S. alopecuroides polysaccharide were studied by orthogonal test. Results The optimal decoloring conditions for S. alopecuroides polysaccharide with H₂O₂ were as following: decolorization temperature of 60 ℃, pH value of 5, addition of 1% H₂O₂, and decolorization reaction for 3 h. Under these conditions, the polysaccharide decolorization rate and polysaccharide retention rate could achieve 29.83% and 71.12% after verification. Conclusion The method with H₂O₂ has good decolorizing effect, and can provide a scientific basis for the production of S. alopecuroides polysaccharide with high quality.

天津市應用基礎及前沿技術研究計劃項目(10JCZDJC21000)