目的 建立多波長HPLC梯度洗脫法同時測定更年樂片中朝藿定C、淫羊藿苷、川續(xù)斷皂苷Ⅵ和大花雙參苷A的方法.方法 依利特C₁₈柱(250 mm×4.6 mm,5 μm);流動相為乙腈–0.1%磷酸溶液,梯度洗脫;檢測波長:270 nm(朝藿定C和淫羊藿苷)、210 nm(川續(xù)斷皂苷Ⅵ)、230 nm(大花雙參苷A);體積流量為1.2 mL/min;柱溫為室溫;進樣量20 μL.結果 朝藿定C、淫羊藿苷、川續(xù)斷皂苷Ⅵ和大花雙參苷A分別在7.14～142.80 μg/mL(r＝0.999 8)、5.64～112.80 μg/mL(r＝0.999 6)、6.35～127.00 μg/mL(r＝0.999 5)、7.90～158.00 μg/mL(r＝0.999 3)與其峰面積呈良好的線性關系;朝藿定C、淫羊藿苷、川續(xù)斷皂苷Ⅵ和大花雙參苷A的平均回收率分別為99.24%、96.93%、97.81%、98.32%,RSD值分別為1.28%、0.94%、1.24%、1.50%.結論 該方法是一種快速、靈敏、準確的分析方法,可作為更年樂片的質量控制方法.

Objective To develop an HPLC method for the simultaneous determination of epimedin C, icariin, asperosaponin Ⅵ, and triplostoside A in Gengnianle Tablets. Methods The analysis was performed on Elite C₁₈ column (250 mm × 4.6 mm, 5 μm) with acetonitrile-0.1% phosphoric acid solution as mobile phases at the flow rate of 1.2 mL/min for gradient elution. Detection with variable wavelength was used, and set at 270 nm for epimedin C and icariin, 210 nm for asperosaponin Ⅵ, and 230 nm for triplostoside A. The column temperature was room temperature with injection volume of 20 μL. Results Epimedin C, icariin, asperosaponin Ⅵ, and triplostoside A had good linearity in the ranges of 7.14—142.80 μg/mL (r = 0.999 8), 5.64—112.80 μg/mL (r = 0.999 6), 6.35—127.00 μg/mL (r = 0.999 5), and 7.90—158.00 μg/mL (r = 0.999 3), respectively. The average recoveries were 99.24%, 96.93%, 97.81%, and 98.32% with RSD 1.28%, 0.94%, 1.24%, and 1.50%, respectively. Conclusion The method is simple, sensitive, and accurate, which can be used in quantity control for Gengnianle Tablets.