目的 研究葛根素對鏈脲佐菌素誘導妊娠期糖尿病大鼠氧化應(yīng)激損傷的保護作用.方法 通過ip 鏈脲佐菌素35mg/kg 制備妊娠期糖尿病大鼠模型.選取64 只模型大鼠并根據(jù)血糖水平隨機分為模型組、葛根素40、80、160 mg/kg 組,另選取16 只同期妊娠的大鼠作為妊娠對照組,并取16 只同齡非妊娠雌性大鼠作為非妊娠對照組.ig 給藥治療,1 次/d,給藥容積為20 mL/kg,連續(xù)給藥2 周.分別于給藥前和給藥第7、14 天測定空腹血糖水平.給藥治療2 周后,測定丙氨酸轉(zhuǎn)氨酶(ALT)、天門冬氨酸轉(zhuǎn)氨酶(AST)、堿性磷酸酶(ALP)活性,檢測血清中丙二醛(MDA)含量及總抗氧化能力(T-AOC)水平;測定肝臟組織中超氧化物歧化酶(SOD)、過氧化氫酶(CAT)活性和MDA 含量.給藥2 周后,通過HE 染色觀察肝臟組織病理形態(tài)學改變.結(jié)果 葛根素160 mg/kg 組大鼠空腹血糖水平較模型組顯著降低(P< 0.05、0.01).與模型組比較,葛根素80、160 mg/kg 組大鼠血清中ALT、AST 活性和MDA 含量均顯著降低(P< 0.05、0.01),葛根素160 mg/kg 組ALP 活性顯著降低(P< 0.01)、T-AOC 水平顯著升高(P< 0.05).與模型組比較,葛根素80、160 mg/kg 組肝臟組織中SOD活性顯著升高(P< 0.05、0.01),MDA 含量顯著降低(P< 0.05、0.01);葛根素160 mg/kg 組CAT 活性顯著升高(P< 0.01).葛根素組大鼠肝臟組織病理形態(tài)學改變明顯改善.結(jié)論 葛根素對鏈脲佐菌素誘導妊娠期糖尿病大鼠氧化應(yīng)激損傷具有保護作用,其作用機制可能與葛根素能夠有效改善抗氧化酶活性、降低氧化應(yīng)激損傷有關(guān).

Objective To investigate protective effects of puerarin against oxidative stress injury in gestational diabetic rats induced by streptozocin. Methods Gestational diabetic model rats were made by ip administration with streptozocin (35 mg/kg). According to blood glucose levels, a total of 64 gestational diabetic rats were randomly divided into model group and puerarin (40, 80, and 160 mg/kg) groups. And other 16 pregnant rats in the same period were selected into the gestation control group, and 16 non-gestation female rats of the same age also were selected into the non-gestation control group. Rats were ig administered with dosage of 20 mL/kg, and the treatment was adopted once daily and lasted for 2 weeks. The level of fasting blood sugar in gestational diabetic rats were determined before treatment and treated for 7 and 14 d. After treatment for 2 weeks, the activities of ALT, AST, and ALP in serum were determined. Also the content of MDA and the level of T-AOC in serum were determined. The activity of SOD, CAT, and the content of MDA in hepatic tissue were determined. Histopathological changes of hepatic tissue were observed by HE staining. Results Compared with model group, the level of fasting blood sugar of puerarin 160 mg/kg group was significantly decreased (P < 0.05, 0.01). Compared with model group, the activities of ALT, AST, and the content of MDA of puerarin 80 and 160 mg/kg groups were significantly decreased (P < 0.05, 0.01), and the activity of ALP in serum of puerarin 160 mg/kg group was significantly decreased (P <0.01), while the level of T-AOC in serum was significantly increased (P < 0.05). Compared with model group, the activity of SOD in hepatic tissue of puerarin 80 and 160 mg/kg groups were significantly increased (P < 0.05, 0.01), but the content of MDA was significantly decreased (P < 0.05, 0.01), and the activity of CAT in hepatic tissue of puerarin 160 mg/kg group was significantly increased (P < 0.01). Histopathological changes of hepatic tissue in puerarin groups were significantly improved. Conclusion Puerarin has protection against oxidative stress injury in gestational diabetic rats induced by streptozocin, which perhaps is related to improvement of the anti-oxidant enzyme activity and depression of oxidative stress.