Objective To evaluate the safety of rabbit corneas with subconjunctival injection of voriconazole, and explore a safe range of antibiotic dose. Methods According to body weights, total 25 healthy rabbits were randomly divided into control group, and voriconazole (50, 100, 200, and 400 μg) groups. The eyes of control group received subconjunctival injection of 0.1 mL balanced salt solution (BBS), and those treatment groups received 0.1 mL injection containing Voriconazole for injection 50, 100, 200, and 400 μg, respectively. Every rabbit received five injections, once daily. Before the administration and 1, 7, and 14 d after the last injection, basic situations of the eyes were observed with slit lamp biomicroscopy and direct ophthalmscopy. Ultrastructures of the corneas were detected in vivo observation 1, 7, and 14 d after the last injection, respectively, and endothelial cell counts and corneal thicknesses were performed at the same time using confocal microscopy. Fourteen days after the last injection, pathological observations were carried out for corneal morphology. Results Subconjunctival injection of voriconazole in the rabbits at doses of 50 and 100 μg, there was no abnormality in corneal cells, but at doses of 200 and 400 μg, the rabbits showed edema cornea, exfoliation of corneal epithelium and abnormities in ultrastructures. However, there was no significant difference in endothelial cell counts and corneal thickness with those of control group at any time points. Conclusion It is safe for corneas of rabbit subconjunctival injection of voriconazole at the dose≤100μg.

天津市衛(wèi)生局科技基金項(xiàng)目(2013KY36)