目的 研究痰熱清注射液對呼吸道合胞病毒感染小鼠肺損傷的保護作用,并探討其作用機制。方法 Balb/c小鼠隨機分為對照組、感染組、利巴韋林(100 mg/kg)組和痰熱清注射液(2、4、8 mL/kg)組,每組10只。呼吸道合胞病毒滴鼻感染后,尾iv給予相應(yīng)劑量的藥物,給藥容積為1 mL/100 g,1次/d,連續(xù)給藥8 d。計算肺指數(shù),測定肺組織懸液lgTCID₅₀,觀察肺組織病理學(xué)改變,測定MDA、GSH含量和IFN-γ活性,檢測T淋巴細胞轉(zhuǎn)化功能和IL-2活性的變化。結(jié)果 經(jīng)痰熱清注射液治療后,與感染組比較,痰熱清注射液2、4、8 mL/kg組小鼠肺指數(shù)、lgTCID₅₀明顯降低(P <0.05),呼吸道合胞病毒損傷小鼠肺組織病理狀態(tài)得到改善。與感染組比較,痰熱清注射液2、4、8 mL/kg組小鼠MDA水平、GSH水平明顯降低,IFN-γ活性明顯升高,SI明顯升高(P <0.05)。結(jié)論 痰熱清注射液對呼吸道合胞病毒感染小鼠的肺損傷具有保護作用,其機制可能與提高機體免疫應(yīng)答能力有關(guān)。

Objective To study the protection of Tanreqing Injection against lung injury in mice induced by respiratory syncytial virus, and discuss its mechanism. Methods Balb/c mice were randomly divided into normal group, viral infection group, ribavirin (100 mg/kg) group, and Tanreqing Injection (2, 4, and 8 mL/kg) groups, and each group had 10 rats. After infected by respiratory syncytial virus, drugs were tail iv administered with volume of 1 mL/100 g, once daily, and all animals were treated for 8 d. Lung index and lgTCID₅₀ were detected, pathological changes of lung tissue were observed, level of MDA, GSH and activity of IFN-γ were determined, and T lymphocyte translation faction and IL-2 activity were detected. Results After treatment of Tanreqing Injection, compared with infection group, lung index and lgTCID₅₀ of Tanreqing Injection (2, 4, and 8 mL/kg) groups were obviously decreased (P < 0.05), and lung injury were improved. Compared with infection group, levels of MDA, GSH were significantly decreased, activity of IFN-γ and SI were significantly increased (P < 0.05). Conclusion Tanreqing Injection has protection against lung injury in mice induced by respiratory syncytial virus, which might be related to improvement of immune response ability.

天津市中醫(yī)藥管理局中醫(yī)﹑中西醫(yī)結(jié)合科研課題(13050)