目的 建立同時測定前列康復(fù)膠囊中大車前苷、朝藿定C、淫羊藿苷的HPLC法。方法 安捷倫Zorbax C₁₈色譜柱(250 mm×4.6 mm,5 μm);以乙腈–0.1%磷酸水溶液為流動相,梯度洗脫;柱溫35 ℃;檢測波長為0～30 min,330 nm(測定大車前苷),30～50 min,270 nm(測定朝藿定C、淫羊藿苷);體積流量1.0 mL/min。結(jié)果 大車前苷、朝藿定C、淫羊藿苷分別在10.08～201.60 ng、18.43～368.60 ng、52.25～1 045.00 ng線性關(guān)系良好;平均回收率分別為100.6%、97.2%、97.2%,RSD值分別為1.5%、1.3%、1.2%。結(jié)論 該方法操作簡單,重復(fù)性好,可有效的控制前列康復(fù)膠囊的質(zhì)量。

Objective To establish a method for simultaneous determination of plantamajoside, epimedin C, and icariin in Qianlie Kangfu Capsules. Methods The determination was carried out on Agilent Zorbax C₁₈ column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile -0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 ml/min. The column temperature was set at 35 ℃ and the detection wavelengths were 330 nm in 0 — 30 min (determination of plantamajoside) and 270 nm in 30 — 50 min (determination of epimedin C and icariin). Results Plantamajoside, epimedin C, and icariin had good linearity in the ranges of 10.08 — 201.60 ng, 18.43 — 368.60 ng, and 52.25 — 1 045.00 ng, respectively. The average recoveries were 100.6%, 97.2%, and 97.2% with RSD 1.5%, 1.3%, and 1.2%, respectively. Conclusion The method is simple and repeatable, which can be applied to the quality control for Qianlie Kangfu Capsules.