目的 利用基因芯片技術(shù)了解阿霉素腎病大鼠基因表達譜及其經(jīng)阿奇霉素干預(yù)后的變化。方法 將81只大鼠隨機分為對照組、模型組、阿奇霉素組,通過尾靜脈注射法建立阿霉素腎病模型,檢測第4、8周時大鼠血生化指標及24 h尿蛋白變化。利用Agilent公司生產(chǎn)的Agilent SurePrint G3 Rat Gene Expression(8×60 k)基因芯片檢測第8周時各組大鼠的腎臟基因表達譜,并利用生物信息學(xué)方法對檢測結(jié)果進行分析。結(jié)果 與模型組比較,阿奇霉素組24 h尿蛋白、總膽固醇、血肌酐均顯著降低(P < 0.01),白蛋白、總蛋白、內(nèi)生肌酐清除率均明顯升高(P < 0.01)。通過芯片檢測得到了各組大鼠的腎臟基因表達譜。與對照組比較,模型組共有185個基因發(fā)生差異性表達;而與模型比較,阿奇霉素組共有824個基因發(fā)生差異性表達。結(jié)論 阿霉素腎病的發(fā)生涉及眾多基因的改變,阿奇霉素干預(yù)治療可以改變其基因表達譜及在基因水平上使其腎組織異常的基因表達實現(xiàn)回歸,對進一步探索阿奇霉素干預(yù)阿霉素腎病的作用機制提供了線索。

Objective To study the gene expression profile of adriamycin nephropathy rats and the changes after the intervention of azithromycin by gene chip technology. Methods Eighty-eight rats were randomly divided into control group, model group, and azithromycin group. The model was established by tail vein injection of adriamycin, and the changes of blood biochemical and 24 h urine protein in rats at 4 th and 8 th week were detected. Agilent SurePrint G3 Rat Gene Expression (8×60k) gene chip were used to detect the gene expression profile, and the results were analyzed by bioinformatics method. Results Eighty-eight rats were randomly divided into control group, model group, and azithromycin group. The model was established by tail vein injection of adriamycin, and the changes of blood biochemical and 24 h urine protein in rats at 4th and 8th week were detected. Agilent SurePrint G3 Rat Gene Expression (8×60 k) gene chip were used to detect the gene expression profile, and the results were analyzed by bioinformatics method. Conclusion The occurrence of adriamycin nephropathy involves the changes of many genes and the gene expression profile can be changed with the intervention of azithromycin, which can provide further evidence to explore the effect of azithromycin on adriamycin induced nephropathy.

天津市應(yīng)用基礎(chǔ)及前沿技術(shù)研究計劃項目(13JCYBJC22300)