目的 探討寧泌泰膠囊的抗炎作用及其作用機(jī)制。方法 采用1%角叉菜膠致小鼠足趾腫脹、2%巴豆油丙酮溶液致小鼠耳腫脹模型,觀察寧泌泰膠囊(0.5、1.5、4.5 g/kg)對(duì)小鼠腫脹的抑制作用。考察25、50、100 μg/mL寧泌泰膠囊加入RAW264.7細(xì)胞作用48、72 h后的細(xì)胞毒性。運(yùn)用雙熒光素酶報(bào)告基因系統(tǒng)檢測(cè)寧泌泰膠囊對(duì)NF-κB轉(zhuǎn)錄活性的影響。結(jié)果 與模型組比較,寧泌泰膠囊(4.5 g/kg)對(duì)角叉菜膠致小鼠足腫脹、巴豆油致小鼠耳腫脹具有明顯的抑制作用(P< 0.05)。寧泌泰膠囊對(duì)RAW264.7細(xì)胞沒(méi)有細(xì)胞毒性。50、100 μg/mL寧泌泰膠囊內(nèi)容物對(duì)NF-κB的轉(zhuǎn)錄激活具有明顯的抑制作用(P< 0.01)。結(jié)論 寧泌泰膠囊具有抗炎作用,其作用機(jī)制可能是通過(guò)NF-κB通路來(lái)抑制炎癥過(guò)程。

Objective To study the anti-inflammatory effect of Ningmitai Capsules and its mechanism. Methods The models of 1% carrageenan-induced mice paw edema and 2% oleum crotonis-induced ear edema were established, and the inhibition of swelling in mice adminisered with Ningmitai Capsules were observed. Cytotoxicity of 25, 50, and 100 μg/mL Ningmitai Capsules on RAW264.7 cells were studied after 48 and 72 h. Dual luciferase report gene system was used to detect the transcriptional activity of NF-κB. Results Compared with model group, Ningmitai Capsules with the dosage of 4.5 g/kg could obviously inhibit the mice foot swelling induced by carrageenan and the ear edema induced by oleum crotonis (P < 0.05). There was no cytotoxicity of Ningmitai Capsules on RAW264.7 cells. Ningmitai Capsules with the concentration of 50 and 100 μg/mL could inhibit the transcriptional activity of NF-κB (P < 0.01). Conclusion Ningmitai Capsules have anti-inflammatory effect, which is related to inhibition of inflammatory processes through NF-κB pathway.