目的 考察甘味顆粒對急性應(yīng)激誘導(dǎo)行為絕望抑郁小鼠行為的影響,并探討其作用機制。方法 ICR小鼠隨機分為對照組、模型組、逍遙丸組、氟西汀組以及甘味顆粒2.11、6.33、19.00 g/kg組,每組15只。模型組和對照組均ig給予蒸餾水,逍遙丸組ig給予逍遙丸1.50 g/kg,氟西汀組ig給予鹽酸氟西汀分散片3.3 mg/kg,甘味顆粒組ig給予甘味顆粒2.11、6.33、19.00 g/kg。1次/d,連續(xù)給藥10 d。各組小鼠懸尾5 min和常溫強迫游泳6 min進行急性應(yīng)激誘導(dǎo)行為絕望抑郁模型,觀察應(yīng)激小鼠的行為學(xué)效應(yīng)。采用酶聯(lián)免疫吸附(ELISA)法檢測小鼠血漿中皮質(zhì)醇水平以及腦組織中單胺類神經(jīng)遞質(zhì)5-羥色胺(5-HT)、去甲腎上腺素(NE)、多巴胺(DA)水平。結(jié)果 與模型組比較,甘味顆粒6.33、19.00 g/kg組的懸尾不動時間顯著減少,差異具有統(tǒng)計學(xué)意義(P<0.05、0.01);甘味顆粒2.11、6.33、19.00 g/kg組的強迫游泳不動時間顯著減少,差異具有統(tǒng)計學(xué)意義(P<0.05、0.01)。與模型組比較,甘味顆粒6.33、19.00 g/kg組血漿皮質(zhì)醇水平均明顯降低,差異具有統(tǒng)計學(xué)意義(P<0.05)。與模型組比較,甘味顆粒6.33、19.00 g/kg組5-HT水平明顯升高,差異具有統(tǒng)計學(xué)意義(P<0.05、0.01),甘味顆粒2.11、6.33、19.00 g/kg組DA、NE水平顯著升高,差異具有統(tǒng)計學(xué)意義(P<0.05、0.01)。結(jié)論 甘味顆粒對急性應(yīng)激誘導(dǎo)行為絕望抑郁小鼠行為具有顯著的改善作用,可能是通過升高單胺類神經(jīng)遞質(zhì)5-HT、DA、NE水平,降低血漿皮質(zhì)醇水平而發(fā)揮其抗抑郁作用。

Objective To investigate the effect of Ganwei Granules on mice with behavioral despair and depression induced by acute stress and explore its mechanism. Methods ICR mice were randomly divided into control group, model group, Xiaoyao Pills group, fluoxetine group, and Ganwei Granules (2.11, 6.33, and 19.00 g/kg) groups, and each group had 15 mice. Mice in control and model groups were ig administered with distilled water, mice in Xiaoyao Pills group were ig administered with Xiaoyao Pills 1.50 g/kg, and mice in fluoxetine group were ig administered with Fluoxetine Hydrochloride Dispersible Tablets 3.3 mg/kg. Mice in Ganwei Granules groups were ig administered with Ganwei Granules 2.11, 6.33, and 19.00 g/kg, respectively. All animals were ig administered once daily, and treated for 10 d. Behavioral despair and depression model induced by acute stress was built by tail suspension for 5 min test and forced swimming for 6 min test. The behavioral effects were observed. The levels of cortisol in plasma and monoamine neurotransmitter serotonin (5-HT), norepinephrine (NE), dopamine (DA) in brain were determined by enzyme-linked immunosorbent assay (ELISA). Results Compared with the model group, the fixed time of tail suspension in Ganwei Granules 6.33 and 19.00 g/kg groups were significantly reduced, and there were differences between two groups (P < 0.05, 0.01), while the fixed time of forced swimming in Ganwei Granules 2.11, 6.33, and 19.00 g/kg groups were significantly reduced, with significant differences between two groups (P < 0.05, 0.01). Compared with the model group, the levels of cortisol in plasma of Ganwei Granules 6.33 and 19.00 g/kg groups were significantly decreased, with significant differences between two groups (P < 0.05). Compared with the model group, the levels of 5-HT in plasma of Ganwei Granules 6.33, 19.00 g/kg groups were significantly increased, and the levels of DA and NE in plasma of Ganwei Granules 2.11, 6.33, and 19.00 g/kg groups were also obviously increased, and there were differences between two groups (P < 0.05, 0.01). Conclusion Ganwei Granules have significant improved effects on mice with behavioral despair and depression induced by acute stress, which may be related to the increase of monoamine neurotransmitter 5-HT, DA, and NE, and the reduction of cortisol in plasma.

山西省實驗動物專項資金項目(2015K06)