目的 建立HPLC同時測定八珍液中的地黃苷A、地黃苷D、去氫土莫酸和茯苓酸的方法。方法 Kromasil C₁₈色譜柱(200 mm×4.6 mm,5 μm);流動相：乙腈–0.05%磷酸溶液,梯度洗脫;檢測波長：203 nm(0～34 min,檢測地黃苷A和地黃苷D)、210 nm(34～65 min,檢測去氫土莫酸和茯苓酸);體積流量1.0 mL/min。結果 地黃苷A、地黃苷D、去氫土莫酸和茯苓酸分別在5.76～115.20、4.05～81.00、6.10～122.00、6.35～127.00 μg/mL線性關系良好;平均回收率分別為98.90%、99.08%、98.71%、97.95%,RSD值分別為1.49%、1.19%、1.63%、0.92%。結論 該方法操作簡便,準確,重復性好,可作為八珍液的質(zhì)量控制方法。

Objective To establish an HPLC method for simultaneous determination of rehmaionoside A, rehmaionoside D, dehydrotumulosic acid, and pachymic acid in Bazhen Solution. Methods The determination was carried out on Kromasil C₁₈ column (200 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile - 0.05% phosphoric acid solution with gradient elution at a flow rate of 1.0 mL/min. The column temperature was set at 30 ℃, and the detection wavelengths were 203 nm in 0 — 34 min (determination of rehmaionoside A and rehmaionoside D) and 210 nm in 34 — 65 min (determination of dehydrotumulosic acid and pachymic acid). Results There were good linear relationships of rehmaionoside A, rehmaionoside D, dehydrotumulosic acid, and pachymic acid in the concentration ranges of 5.76 — 115.20 μg/mL, 4.05 — 81.00 μg/mL, 6.10 — 122.00 μg/mL, and 6.35 — 127.00 μg/mL. The average recoveries were 98.90%, 99.08%, 98.71%, and 97.95% with RSD 1.49%, 1.19%, 1.63%, and 0.92%, respectively. Conclusion The method is simple, accurate, and repeated, which can be used in quantity control for Bazhen Solution.