目的 探討瑞舒伐他汀對(duì)人神經(jīng)膠質(zhì)瘤U251細(xì)胞增殖和凋亡的影響。方法 體外培養(yǎng)人神經(jīng)膠質(zhì)細(xì)胞瘤U251細(xì)胞,分別采用5、10、20 μmol/L瑞舒伐他汀進(jìn)行干預(yù),培養(yǎng)24、48、72、96 h,采用MTT比色法檢測(cè)細(xì)胞增殖活性,流式細(xì)胞儀檢測(cè)U251細(xì)胞周期的變化。結(jié)果 處理96 h后,570 nm處吸光度(A_{570 nm})值變化最明顯,瑞舒伐他汀每個(gè)濃度組A_{570 nm}值與對(duì)照組比較均顯著下降(P<0.01)。瑞舒伐他汀處理細(xì)胞48 h后,與對(duì)照組比較,G0/G1期細(xì)胞增多,S期和G2/M期細(xì)胞減少,且瑞舒伐他汀濃度越大,該作用越強(qiáng)。使用不同濃度瑞舒伐他汀處理48~72 h能誘導(dǎo)U25l細(xì)胞凋亡。隨著瑞舒伐他汀濃度的增加、作用時(shí)間的延長(zhǎng),凋亡峰更加明顯,并呈濃度-效應(yīng)和濃度-時(shí)間相關(guān)性。結(jié)論 瑞舒伐他汀對(duì)膠質(zhì)瘤U251細(xì)胞增殖具有一定的抑制作用,可以誘導(dǎo)膠質(zhì)瘤U251細(xì)胞凋亡。

Objective To explore the influence of rosuvastatin on proliferation and apoptosis of human glioma U251 cells.Methods Human glioma U251 cells were cultured in vitro, and treated by rosuvastatin with the concentrations of 5, 10, and 20 μmol/L. After cultivation for 24, 48, 72, and 96 h, proliferation activities and cell cycle distribution were determined by MTT colorimetric assay and flow cytometer.ResultsCompared with the control group, A_{570 nm} values of rosuvastatin groups had obvious changes, and were significantly decreased after being treated for 96 h (P<0.01). Compared with the control group, cells in G0/G1 phase were increased, but those in S and G2/M phases were decreased after being treated for 96 h by rosuvastatin, which had dose-effect dependence. Apoptosis of human glioma U251 cells were induced by rosuvastatin with various concentrations treated for 48-72 h. With the increase of the concentrations and action time of rosuvastatin, the apoptotic peaks were more obvious, and showed concentration-effect dependent and time-concentration dependent.Conclusion Rosuvastatin can inhibit the proliferation of human glioma U25l cells, and induced their apoptosis.

河北省科技計(jì)劃項(xiàng)目(132777107D)