目的 考察丹參多酚酸對糖尿病大鼠血液流變學(xué)的改善作用,并探討其作用機制。方法 SD大鼠隨機分為對照組和模型組,其中對照組10只,模型組30只。進行糖尿病模型誘導(dǎo)后,造模成功的大鼠隨機分為模型組以及丹參多酚酸1.79、5.26 mg/kg組,每組10只。丹參多酚酸組ip注射用丹參多酚酸鹽1.79、5.26 mg/kg(以丹參乙酸鎂計),模型組、對照組ip同體積蒸餾水,給藥容積均為2.76 mL/kg,1次/d,連續(xù)給藥14 d。取血測定血液流變學(xué)指標和紅細胞膜表面生物學(xué)特性指標。結(jié)果 與模型組比較,丹參多酚酸1.79、5.26 mg/kg組全血黏度3、全血黏度50、紅細胞電泳時間、血沉方程K值均顯著降低(P<0.05、0.01)。與模型組比較,丹參多酚酸1.79、5.26 mg/kg組唾液酸含量顯著升高(P<0.05),丹參多酚酸5.26 mg/kg組Na⁺-K⁺-ATP酶活性、SOD水平均顯著升高(P<0.05)。結(jié)論 丹參多酚酸鹽通過增加紅細胞膜表面唾液酸含量、提高紅細胞膜抗氧化能力來改善糖尿病大鼠的血液流變學(xué)特性。

Objective To study the improvement of salvianolic acids on hemorheology of diabetic rats and to explore its mechanism.Methods SD rats were randomly divided into control and model groups. And there were 10 rats in the control group, and 30 rats in the model group. Diabetes model were induced. Diabetic rats were randomly divided into model group and salvianolic acids (1.79 and 5.26 mg) groups, and each group had 10 rats. Rats in salvianolic acids groups were ip administered with Depsides Salts from Salvia miltiorrhiza in Lyophilizied Powder for injection 1.79 and 5.26 mg (magnesium acetate as index), and those in control and model groups were ip administered with the same volume of normal saline. The volume was 2.76 mL/kg, the treatment were carried out once daily, and lasted for 14 d. The indexes of hemorheology and surface characteristics of erythrocyte membrane were determined.Results Compared with the model group, the whole blood viscosity 3, whole blood viscosity 50, erythrocyte electrophoretic time, and ESR equation K values in salvianolic acids (1.79 and 5.26 mg/kg) groups were significantly decreased (P < 0.05, 0.01). Compared with the model group, the contents of sialic acid in salvianolic acids (1.79 and 5.26 mg/kg) groups were significantly increased (P < 0.05), and Na⁺-K⁺-ATP enzyme activities and SOD levels in salvianolic acids (5.26 mg/kg) group were significantly increased (P < 0.05).Conclusion Salvianolic acids can increase sialic acid contents of red cell surface, enhance antioxidant capacity of erythrocyte membrane, and improve hemorheology characteristics of diabetic rats.

黑龍江省青年科學(xué)基金資助項目(QC2015122)