目的 研究元胡止痛滴丸對(duì)大鼠實(shí)驗(yàn)性偏頭痛的鎮(zhèn)痛作用及其作用機(jī)制。方法 Wistar大鼠隨機(jī)分為對(duì)照組、模型組、正天丸(3.0 g/kg)組以及元胡止痛滴丸0.4、0.8、1.6 g生藥/kg組,每組10只,ig給藥,給藥體積0.5 mL/100 g,1次/d,連續(xù)給藥3 d。對(duì)照組和模型組給予等體積的去離子水。末次給藥后30 min,除對(duì)照組外,其余各組大鼠sc硝酸甘油注射液10 mg/kg,復(fù)制大鼠實(shí)驗(yàn)性偏頭痛模型。立即記錄大鼠搔頭潛伏期以及60 min內(nèi)的搔頭次數(shù),計(jì)算搔頭潛伏期延長率和搔頭次數(shù)抑制率。測定大鼠腦組織和血清中多巴胺(DA)、5-羥色胺(5-HT)、β-內(nèi)啡肽(β-EP)、降鈣素基因相關(guān)肽(CGRP)、NO水平。結(jié)果 與模型組比較,元胡止痛滴丸0.8、1.6 g生藥/kg組偏頭痛大鼠的搔頭次數(shù)顯著減少(P<0.05、0.01),并呈劑量相關(guān)性。與模型組比較,元胡止痛滴丸0.8 g生藥/kg組DA、NO水平顯著降低(P<0.05),1.6 g生藥/kg組5-HT、DA、NO水平顯著降低(P<0.01、0.001),β-EP水平顯著升高(P<0.05),但對(duì)CGRP水平基本無影響。結(jié)論 元胡止痛滴丸在0.8~1.6 g生藥/kg對(duì)大鼠實(shí)驗(yàn)性偏頭痛具有顯著的鎮(zhèn)痛作用,其作用機(jī)制可能與調(diào)節(jié)5-HT、NO、DA、β-EP等與疼痛相關(guān)的血管活性物質(zhì)及神經(jīng)遞質(zhì)的水平有關(guān)。

Objective To study analgesic effect of Yuanhu Zhitong Dropping Pills on experimental migraine in rats induced by nitroglycerin and explore its mechanism.Methods Wistar rats were randomly divided into control group, model group, Zhengtian Pills (3.0 g/kg) group, and Yuanhu Zhitong Dropping Pills (0.4, 0.8, and 1.6 crude drug g/kg) groups, and each group had 10 rats. Rats were ig administered with corresponding drugs 0.5 mL/100 g except the control group and model group, once daily, and treated for 3 d. Rats except in the control group were sc administered with Nitroglycerin Injection 10 mg/kg to make migraine model after last administration 30 min. The latent periods and frequencies in 60 min of scratching head reaction were observed, and the prolonged rate of latent period and inhibitory rate of scratching head were calculated. The levels of DA, 5-HT, β-EP, CGRP, and NO in brain tissues and serum were determined.Results Compared with the model group, the frequency of scratching head of rats in Yuanhu Zhitong Dropping Pills (0.8 and 1.6 crude drug g/kg) groups were significantly reduced (P< 0.05, 0.01), and showed dose dependent. Compared with the model group, levels of DA and NO in Yuanhu Zhitong Dropping Pills (0.8 crude drug g/kg) group were markedly decreased (P< 0.05). The levels of DA and NO in Yuanhu Zhitong Dropping Pills (1.6 crude drug g/kg) group were significantly decreased (P< 0.01, 0.001), but the level of β-EP was significantly increased (P< 0.05), and there was no difference on the level of CGRP between the model group and Yuanhu Zhitong Dropping Pills (1.6 crude drug g/kg) group.Conclusion Yuanhu Zhitong Dropping Pills has significantly analgesic effect on experimental migraine model of rats, which may be related to the levels of vasoactive substances and neurotransmitters related to pain, such as 5-HT, NO, DA, and β-EP.