目的 建立HPLC波長切換法同時測定養(yǎng)陰降壓膠囊中馬兜鈴酸I、氧化芍藥苷、芍藥內(nèi)酯苷和芍藥苷。方法 采用HPLC法，Venusil MP C₁₈色譜柱（250 mm×4.6 mm，5 μm）；流動相：[甲醇-乙腈（2∶1）]-0.02%磷酸溶液，梯度洗脫；0~16 min在390 nm波長下檢測馬兜鈴酸Ⅰ，16~40 min時在230 nm波長下檢測氧化芍藥苷、芍藥內(nèi)酯苷和芍藥苷；體積流量1.0 mL/min；柱溫35 ℃；進(jìn)樣量10 μL。結(jié)果 馬兜鈴酸I、氧化芍藥苷、芍藥內(nèi)酯苷和芍藥苷質(zhì)量濃度分別在4.72~94.40（r=0.999 8）、3.95~79.00（r=0.999 9）、6.39~127.80（r=0.999 9）、19.81~396.20 μg/mL（r=0.999 1）與峰面積呈良好的線性關(guān)系；平均回收率分別為97.47%、99.09%、100.09%、98.88%，RSD值分別為1.19%、1.60%、0.84%、0.65%。結(jié)論 所建立的方法簡便，重復(fù)性好，為有效控制養(yǎng)陰降壓膠囊的質(zhì)量提供了依據(jù)。

Objective To develop an HPLC-wavelength switching method for simultaneous determination of aristolochic acid I, oxypaeoniflorin, alibiflorin, and paeoniflorin in Yangyin Jiangya Capsules. Method HPLC method was adopted. The determination was carried out on Venusil MP C₁₈ chromatographic column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of methanol-acetonitrile (2:1) and 0.02% phosphoric acid solution with gradient elution. The detection wavelengths were set at 390 nm in 0-16 min (determination of aristolochic acid I) and 230 nm in 16-40 min (determination of oxypaeoniflorin, alibiflorin, and paeoniflorin). The flow rate was 1.0 mL/min, temperature of column was set at 35℃, and volume of injection was 20 μL. Results There were good linear relationships of aristolochic acid I, oxypaeoniflorin, alibiflorin, and paeoniflorin in the concentration ranges of 4.72-94.40 μg/mL (r=0.999 8), 3.95-79.00 μg/mL (r=0.999 9), 6.39-127.80 μg/mL (r=0.999 9), and 19.81-396.20 μg/mL (r=0.999 1) between peak areas, respectively. The average recoveries were 97.47%, 99.09%, 100.09%, and 98.88% with RSD 1.19%, 1.60%, 0.84%, and 0.65%, respectively. Conclusion The established method is simple and has good repeatability which can be used in quantity control for Yangyin Jiangya Capsules.