18色譜柱(250 mm×4.6 mm,5 μm);流動(dòng)相:0.05%磷酸-乙腈,梯度洗脫;檢測(cè)波長(zhǎng):327 nm;體積流量:0.8 mL/min;柱溫:35℃;進(jìn)樣量:10 μL。結(jié)果 綠原酸、3,5-二咖啡??鼘幩?、木犀草素-7-O-β-D-葡萄糖苷、芹菜素-7-O-β-D-葡萄糖苷和蒙花苷分別在0.105~2.100 μg(r=0.999 9)、0.165~3.300 μg(r=0.999 8)、0.081~1.620 μg(r=0.999 7)、0.060~1.200 μg(r=0.999 6)、0.317~6.340 μg(r=0.999 5)與峰面積呈良好的線性關(guān)系,平均加樣回收率分別為99.19%、99.54%、99.34%、98.83%和99.26%,RSD值分別為0.87%、1.04%、1.06%、1.29%、0.91%。結(jié)論 該方法簡(jiǎn)便、準(zhǔn)確、重復(fù)性好,可用于野菊花栓中有機(jī)酸和黃酮類多成分的質(zhì)量控制。;Objective To develop an HPLC-DAD method for simultaneous determination of chlorogenic acid, 3,5-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucoside, and linarin in Yejuhua Suppository. Methods HPLC-DAD was used. Chromatographic separation was performed on a Thermo Hypersil GOLD C18 (250 mm×4.6 mm, 5 μm), and the mobile phase was acetonitrile-0.05% phosphoric acid with gradient elution. The detection wavelengths were set at 327 nm, flow rate was 0.8 mL/min, the column temperature was maintained at 35℃, and injection volume was 10 μL. Results Chlorogenic acid, 3,5-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucoside, and linarin had good linearity in the ranges of 0.105-2.100 μg (r=0.999 9), 0.165-3.300 μg (r=0.999 8), 0.081-1.620 μg (r=0.999 7), 0.060-1.200 μg (r=0.999 6), and 0.317-6.340 μg (r=0.999 5), respectively. The average recoveries were 99.19%, 99.54%, 99.34%, 98.83%, and 99.26%, with RSD of 0.87%, 1.04%, 1.06%, 1.29%, and 0.91%, respectively. Conclusions The method is convenient, accurate and reproducible which can be applied to the quality control of organic acids and flavonoids in Yejuhua Suppository."/>