目的 建立高效液相色譜法聯(lián)合二極管陣列檢測(cè)器（HPLC-DAD）法同時(shí)測(cè)定野菊花栓中綠原酸、3,5-二咖啡?？鼘幩?、木犀草素-7-O-β-D-葡萄糖苷、芹菜素-7-O-β-D-葡萄糖苷和蒙花苷。方法 采用HPLC-DAD法，Thermo Hypersil GOLD C₁₈色譜柱（250 mm×4.6 mm，5 μm）；流動(dòng)相：0.05%磷酸-乙腈，梯度洗脫；檢測(cè)波長(zhǎng)：327 nm；體積流量：0.8 mL/min；柱溫：35℃；進(jìn)樣量：10 μL。結(jié)果 綠原酸、3,5-二咖啡?？鼘幩帷⒛鞠菟?7-O-β-D-葡萄糖苷、芹菜素-7-O-β-D-葡萄糖苷和蒙花苷分別在0.105～2.100 μg（r=0.999 9）、0.165～3.300 μg（r=0.999 8）、0.081～1.620 μg（r=0.999 7）、0.060～1.200 μg（r=0.999 6）、0.317～6.340 μg（r=0.999 5）與峰面積呈良好的線性關(guān)系，平均加樣回收率分別為99.19%、99.54%、99.34%、98.83%和99.26%，RSD值分別為0.87%、1.04%、1.06%、1.29%、0.91%。結(jié)論 該方法簡(jiǎn)便、準(zhǔn)確、重復(fù)性好，可用于野菊花栓中有機(jī)酸和黃酮類多成分的質(zhì)量控制。

Objective To develop an HPLC-DAD method for simultaneous determination of chlorogenic acid, 3,5-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucoside, and linarin in Yejuhua Suppository. Methods HPLC-DAD was used. Chromatographic separation was performed on a Thermo Hypersil GOLD C₁₈ (250 mm×4.6 mm, 5 μm), and the mobile phase was acetonitrile-0.05% phosphoric acid with gradient elution. The detection wavelengths were set at 327 nm, flow rate was 0.8 mL/min, the column temperature was maintained at 35℃, and injection volume was 10 μL. Results Chlorogenic acid, 3,5-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucoside, and linarin had good linearity in the ranges of 0.105-2.100 μg (r=0.999 9), 0.165-3.300 μg (r=0.999 8), 0.081-1.620 μg (r=0.999 7), 0.060-1.200 μg (r=0.999 6), and 0.317-6.340 μg (r=0.999 5), respectively. The average recoveries were 99.19%, 99.54%, 99.34%, 98.83%, and 99.26%, with RSD of 0.87%, 1.04%, 1.06%, 1.29%, and 0.91%, respectively. Conclusions The method is convenient, accurate and reproducible which can be applied to the quality control of organic acids and flavonoids in Yejuhua Suppository.