目的 鑒定生川烏配伍白蘞、白及的入血成分，比較配伍前后血中移行成分的變化特征。方法 大鼠ig給予生川烏配伍白蘞、白及提取物，收集血漿樣品并采用UPLC-QTOF/MS法鑒定入血成分，由半定量分析比較配伍前后血中移行成分的變化。結(jié)果 檢測(cè)配伍組20個(gè)成分，均為來自于生川烏的原形成分，配伍組中雙酯型生物堿的相對(duì)質(zhì)量分?jǐn)?shù)均顯著降低，單酯型生物堿中苯甲酰烏頭原堿的相對(duì)質(zhì)量分?jǐn)?shù)顯著降低，醇胺型生物堿中宋果靈的相對(duì)質(zhì)量分?jǐn)?shù)顯著降低，其他沒有顯著性變化。結(jié)論 生川烏配伍白蘞、白及能夠改變其血中移行成分的體內(nèi)過程。

Objective To identify the absorbed constituents in rat plasma after administered crude Aconiti Radix in compatibility with Ampelopsis Radix or Bletillae Rhizoma, and to compare changes of the absorbed constituents in plasma before and after compatibility. Methods Rats were given extract of crude Aconiti Radix in compatibility with Ampelopsis Radix or Bletillae Rhizoma by intragastric administration, and the plasma samples were collected and analyzed by UPLC-QTOF/MS method. Changes of the migrating components in rat plasma before and after compatibility were compared by semi-quantitative analysis.Results Twenty kinds of components were detected in rat plasma, which were all prototype components from Aconiti Radix. After compatibility, the contents of diester-type alkaloids were significantly decreased, and the contents of benzoylaconitine (monoester-type alkaloid) and songorine (alcohol amine-type alkaloid) were significantly decreased, but there were no significant changes in other components. Conclusion Compatibility of Aconiti Radix with Ampelopsis Radix or Bletillae Rhizoma could change the in vivo process of absorbed constituents in plasma.

國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃項(xiàng)目（2011CB505300，2011CB505302）；天津市高等學(xué)?？萍及l(fā)展基金計(jì)劃項(xiàng)目（20140208）