目的 比較豬、牛、羊膽汁制膽南星中膽酸類成分及其對發(fā)熱小鼠體溫的影響，為規(guī)范膽南星發(fā)酵工藝提供依據。方法 分別采用豬、牛、羊膽汁與天南星生品發(fā)酵制備膽南星。采用HPLC-CAD法檢測3種膽南星中膽酸類成分膽酸、去氧膽酸、豬去氧膽酸、鵝去氧膽酸。以干酵母制備小鼠發(fā)熱模型，觀察豬、牛、羊膽汁制膽南星對發(fā)熱小鼠體溫的影響。結果 不同膽汁制膽南星中膽酸類成分存在差異，牛膽汁制膽南星中膽酸類成分質量分數(shù)最高，膽酸、豬去氧膽酸、鵝去氧膽酸、去氧膽酸的質量分數(shù)分別為0.035 9%、0.843 4%、2.260 2%、0.037 2%。豬膽汁制膽南星中鵝去氧膽酸和去氧膽酸升高，膽酸和豬去氧膽酸降低；羊膽汁制膽南星中膽酸類成分除去氧膽酸外均降低。不同膽汁制膽南星對發(fā)熱小鼠均有一定的清熱作用，豬膽汁制膽南星組小鼠體溫先升高后降低，且與模型組比較差異有顯著性，牛膽汁制膽南星亦表現(xiàn)出清熱作用，但與模型組比較無顯著性差異。羊膽汁制膽南星清熱作用較弱。結論 綜合比較豬、牛、羊膽汁制膽南星中膽酸類成分質量分數(shù)和清熱作用，作為制備膽南星的輔料，牛膽汁、豬膽汁優(yōu)于羊膽汁。

Objective To compare bile acids contents in different Arisaema cum Bile processed with pig's bile, bilis bovina, and sheep bile and discuss the differences of temperature changes in fever mice, and provide the basis for Arisaema cum Bile fermentation process. Methods Arisaema cum Bile were prepared by fermentation on Arisaematis Rhizoma with pig's bile, bilis bovina, and sheep bile. HPLC-CAD method was used to determine the bile acid composition (cholic acid, deoxidationcholic acid, hyodeoxycholic acid, and chenodeoxycholic acid) in three kinds of Arisaema cum Bile. The fever model of mice was established with dry yeast, and the effects of clearing the heat on the models were observed and compared.Results There were significant differences of bile acids among three kinds of Arisaema cum Bile, and bile acids composition contents were the highest in Arisaema cum Bile processed with bilis bovina, and cholic acid, hyodeoxycholic acid, chenodeoxycholic acid, and deoxidationcholic acid were 0.035 9%, 0.843 4%, 2.260 2%, and 0.843 4%, respectively. Contents of chenodeoxycholic acid and deoxidationcholic acid were decreased in Arisaema cum Bile processed with pig's bile, but those of cholic acid and hyodeoxycholic acid were significantly decreased. Contents of bile acids except deoxidationcholic acid in Arisaema cum Bile processed with sheep bile were significantly decreased. The three kinds of Arisaema cum Bile samples all showed clearing heat function. Mice temperature in Arisaema cum Bile processed with pig's bile group increased first and then decreased. Compared with model group, Arisaema cum Bile processed with bilis bovina also showed clearing heat effects, but there was no significant difference. Arisaema cum Bile processed with sheep bile had weak clearing heat function. Conclusion Both the contents of bile acids and the effects of clearing heat are different among Arisaema cum Bile processed with pig's bile, bilis bovina, and sheep bile, and pig's bile and bilis bovina are better than sheep bile as Arisaema cum Bile processing adjuvants.

公益性行業(yè)科研專項經費項目（201507004-03）