目的 探討槲皮素對人胃癌MKN45細胞凋亡的影響及其作用機制。方法 取對數生長期的MKN45細胞分為對照組和20、40、80 μmol/L槲皮素組。應用MTT比色法測定細胞活性，Hoechst染色、流式細胞技術檢測細胞凋亡，比色法檢測凋亡相關因子Caspase-3、Caspase-9表達。結果 槲皮素能顯著抑制MKN45細胞增殖，促進細胞凋亡，升高Caspase-3、Caspase-9基因表達水平。結論 槲皮素可通過增強Caspase-3、Caspase-9基因表達誘導人胃癌MKN45細胞凋亡。

Objective To investigate the effect and mechanism of quercetin on MKN45 cells. Methods MKN45 cells were divided into four group: negative control group and quercetin groups (20, 40, and 80 μmol/L). Cell viability was measured by MTT colorimetric assay, apoptosis was detected by Hoechst staining and flow cytometry assay, and expression of apoptosis-related factors Caspase-3 and Caspase-9 were tested by colorimetric detection. Results Quercetin could obviously inhibit MKN45 cell proliferation and promote apoptosis, and could increase Caspase-3 and Caspase-9 gene expression levels. Conclusion Quercetin can induce gastric carcinoma MKN45 cells apoptosis by enhancing the expression of Caspase-3 and Caspase-9 gene.

天津市中醫(yī)中西醫(yī)結合科研課題（2015054）