目的 評價坤泰膠囊對初老雌性小鼠戊巴比妥鈉催眠作用的影響及其促睡眠機(jī)制。方法 采用翻正反射試驗考察坤泰膠囊0.8 g/kg對閾劑量戊巴比妥鈉誘導(dǎo)的小鼠入睡潛伏期和睡眠時間的影響；采用RT-PCR技術(shù)檢測坤泰膠囊0.8 g/kg對鹽誘導(dǎo)激酶3（Sik3）和G蛋白偶聯(lián)雌激素受體（Gper1）基因mRNA在不同睡眠狀態(tài)下表達(dá)情況的影響。結(jié)果 坤泰膠囊0.8 g/kg可以顯著延長閾劑量戊巴比妥鈉誘導(dǎo)小鼠的睡眠時間（P<0.05），縮短睡眠潛伏期（P<0.05）；坤泰膠囊0.8 g/kg可升高視前區(qū)內(nèi)Sik3 mRNA在6 h急性睡眠剝奪后的表達(dá)（P<0.05）。坤泰膠囊0.8 g/kg可一致性地降低大腦視前區(qū)和腹側(cè)紋狀體以及卵巢內(nèi)Gper1 mRNA的表達(dá)，坤泰膠囊奪眠組顯著降低腹側(cè)紋狀體和卵巢的雌激素受體基因Gper1 mRNA的表達(dá)（P<0.05）。結(jié)論 坤泰膠囊可增強(qiáng)初老雌性小鼠的戊巴比妥鈉催眠作用，其睡眠改善功效可能是通過提高Sik3和降低Gper1的表達(dá)而發(fā)揮作用。

Objective To study the effect of Kuntai Capsules on sleep induced by pentobarbital sodium in primary and old female mice and it's mechanism. Methods The effect of Kuntai Capsules 0.8 g/kg on latency to persistent sleep and sleep time induced by pentobarbital sodium at threshold dose in mice and it's possible mechanism by righting reflection test. The effect of Kuntai Capsules 0.8 g/kg on expression of Sik3 and Gper1 mRNA in different sleep states were detected by RT-PCR method. Results Kuntai Capsules 0.8 g/kg could significantly prolong sleep time induced by pentobarbital sodium at threshold dose in mice (P<0.05), and decrease sleep latency (P<0.05). Kuntai Capsules 0.8 g/kg could increase the expression of Sik3 mRNA in preoptic area after 6 h of acute sleep deprivation (P<0.05). Kuntai Capsules 0.8 g/kg could coincidently decrease the expression of Gper1 mRNA in preoptic area, ventral striatum, and ovary. Kuntai Capsules sleep-deprived group could significantly decrease the expression of Gper1 mRNA in ventral striatum and ovary (P<0.05). Conclusion Kuntai Capsules can increase sleep induced by pentobarbital sodium in primary and old female mice, and the sleep improvement effect is likely mediated by upregulating Sik3 and downregulating Gper1 mRNA expression.

R966;R285.5