[關(guān)鍵詞]
[摘要]
目的 探究塞來昔布通過上調(diào)Cyclin D1基因甲基化水平對食管癌細胞增殖和凋亡的作用及機制。方法 將細胞分為對照、轉(zhuǎn)染和塞來昔布組,分別轉(zhuǎn)染陰性對照載體、Cyclin D1過表達載體和Cyclin D1轉(zhuǎn)染后給予60 μmol/L塞來昔布。采用MTT法、流式細胞術(shù)分別檢測細胞增殖、細胞周期和細胞凋亡率的變化,Western blotting法檢測細胞周期和細胞凋亡相關(guān)蛋白表達水平;甲基化特異性PCR(MS-PCR)、qRT-PCR法用于檢測Cyclin D1甲基化特異性擴增片段和Cyclin D1 mRNA表達水平。結(jié)果 塞來昔布能夠抑制Cyclin D1誘導的細胞體外增殖,阻滯細胞周期向S期轉(zhuǎn)化,并促進食管癌細胞凋亡;MS-PCR結(jié)果顯示塞來昔布能夠上調(diào)Cyclin D1基因甲基化水平,在轉(zhuǎn)錄水平抑制細胞內(nèi)Cyclin D1 mRNA的表達。結(jié)論 塞來昔布能夠通過上調(diào)Cyclin D1基因甲基化水平發(fā)揮抑制食管癌細胞增殖、促進其體外凋亡的作用。
[Key word]
[Abstract]
Objective To investigate the effects and mechanism of celecoxib on the cell proliferation and apoptosis by regulating methylation status of Cyclin D1 in esophageal carcinoma cells. Methods Cells were divided into control group, transfected group and celecoxib group. Cells in the control group were transfected with negative control vector while cells in the transfected group and celecoxib group were transfected with Cyclin D1-overexpression vector. The celecoxib group was treated with 60 μmol/L celecoxib solution after transfection. MTT and flow cytometry were used to detect the cell proliferation capacity, cell cycle distribution, and cell apoptotic rates in vitro. Western blotting was used to measure the expression levels of related proteins. MS-PCR and qRT-PCR were used to detect the Cyclin D1 promoter region methylation level and Cyclin D1 mRNA expression level. Results Celecoxib can inhibit cell proliferation in vitro, which was promoted by Cyclin D1 overexpression, block cell cycle transition into S phase and promote cell apoptosis. MS-PCR showed that celecoxib could up-regulate the methylation level of Cyclin D1 gene and suppressed Cyclin D1 mRNA expression at transcriptional level. Conclusion Celecoxib can inhibit cell proliferation and promote apoptotic rate of esophageal cancer cells through up-regulating the methylation level of Cyclin D1.
[中圖分類號]
R966
[基金項目]
河南省科技計劃發(fā)展項目(202102310408)