目的 探討復(fù)方木雞顆粒聯(lián)合順鉑對(duì)人肝癌HepG2細(xì)胞增殖、凋亡的影響及其潛在的作用機(jī)制。方法 體外培養(yǎng)人肝癌HepG2細(xì)胞，取對(duì)數(shù)生長(zhǎng)期細(xì)胞，將其消化后隨機(jī)分為對(duì)照組，復(fù)方木雞顆粒高、低（10、5 mg/mL）劑量組，順鉑組（1 mg/mL）和復(fù)方木雞顆粒（5 mg/mL）聯(lián)合順鉑（1 mg/mL）組，采用CCK-8法檢測(cè)細(xì)胞增殖情況，采用Hoehcst染色法檢測(cè)細(xì)胞凋亡情況，采用免疫熒光聯(lián)合激光共聚焦分別檢測(cè)各組細(xì)胞中凋亡相關(guān)蛋白B淋巴細(xì)胞瘤-2（Bcl-2）、Bcl-2相關(guān)X蛋白（Bax）、蛋白天冬氨酸蛋白水解酶-9（Caspase-9）、細(xì)胞色素C氧化酶（Cyt-C）表達(dá)水平。結(jié)果 與對(duì)照組比較，復(fù)方木雞顆粒10、5 mg/mL組及順鉑組、聯(lián)合組的細(xì)胞活力顯著降低（P<0.01），細(xì)胞凋亡顯著增加，細(xì)胞內(nèi)Bcl-2蛋白表達(dá)顯著下調(diào)（P<0.05、0.01），而B(niǎo)ax、Caspase-9、Cyt-C蛋白顯著上調(diào)（P<0.05、0.01），且聯(lián)合組在抑制人肝癌HepG2細(xì)胞增殖、促進(jìn)其凋亡、調(diào)控凋亡相關(guān)蛋白表達(dá)的作用效果最佳。結(jié)論 復(fù)方木雞顆粒可顯著抑制HepG2細(xì)胞增殖并促進(jìn)其凋亡，其與順鉑聯(lián)合起到協(xié)同增效的效果，其作用機(jī)制可能與下調(diào)人肝癌HepG2細(xì)胞內(nèi)抑凋亡蛋白Bcl-2，上調(diào)促凋亡蛋白Bax、Caspase-9、Cyt-C表達(dá)水平有關(guān)。

Objective To discuss the effects of Compound Muji Granules combined with cisplatin on proliferation and apoptosis of human hepatoma HepG2 and its potential mechanism. Methods HepG2 cells were cultured in vitro, and then were divided into the control group, Compound Muji Granules high and low (10 and 5 mg/mL) groups, cisplatin group (1 mg/mL), Compound Muji Granules (5 mg/mL) + cisplatin (1 mg/mL) group. The cell proliferation inhibition was detected by CCK-8. Hoehcst staining was used to detect the apoptosis of HepG2 cells, and the immunofluorescent staining was used to detect the protein expressions of Bcl-2, Bax, Caspase 9, and Cyt C in HepG2 cells. Results Compared with the control group, the cell viability of Compound Muji Granules 10 and 5 mg/mL groups, cisplatin group, and Compound Muji Granules + cisplatin group was significantly reduced (P < 0.01), while the apoptosis of HepG2 cells was increased, and the relative expression of Bcl-2 protein was decreased, but the relative expression of Bax, Caspase 9, and Cyt C protein was significantly increased (P < 0.05, 0.01). And Compound Muji Granules + cisplatin group had the best effect on inhibiting the proliferation, promoting the apoptosis and regulating the expression of apoptosis-related proteins of HepG2 cells. Conclusion Compound Muji Granules inhibits the proliferation and promotes the apoptosis of HepG2 cells, and the effect enhanced while combined with cisplatin by reducing Bcl-2 and increasing Bax, Caspase-9, and Cyt C.

R966

湖南省自然科學(xué)基金科衛(wèi)聯(lián)合項(xiàng)目（2018JJ6078）