A、毛蕊異黃酮葡萄糖苷和芒柄花素。方法 采用Kromasil C18色譜柱（250 mm×4.6 mm，5μm）；流動相：乙腈-0.1%甲酸溶液，梯度洗脫；檢測波長分別為238 nm（0~21.0 min檢測雞矢藤次苷甲酯、車葉草苷酸和車葉草苷）、280 nm（21.0~40.0 min檢測丹參素、丹酚酸B和丹參酮ⅡA）和254 nm（40.0~55.0 min檢測毛蕊異黃酮葡萄糖苷和芒柄花素）；體積流量：1.0 mL/min；柱溫：30℃；進(jìn)樣量為10 μL。結(jié)果 雞矢藤次苷甲酯、車葉草苷酸、車葉草苷、丹參素、丹酚酸B、丹參酮ⅡA、毛蕊異黃酮葡萄糖苷和芒柄花素分別在1.49~37.25、0.76~19.00、1.07~26.75、0.99~24.75、12.61~315.25、2.43~60.75、0.69~17.25、1.86~46.50μg/mL線性關(guān)系良好（r ≥ 0.999 1）；各成分平均加樣回收率分別為98.44%、98.12%、99.36%、97.44%、100.00%、99.19%、96.98%、98.93%，RSD值分別為0.71%、1.47%、0.98%、1.35%、0.85%、1.13%、1.02%、1.29%。結(jié)論 該方法操作簡便、重復(fù)性好，可用于乙肝寧顆粒中多指標(biāo)性成分的質(zhì)量控制。;Objective To establish an HPLC method for simultaneous determination of feretoside, asperulosidic acid, asperuloside, danshensu, salvianolic acid B, tanshinone ⅡA, calycosin 7-O-β-D-glucopyranoside and formononetin in Yiganning Granules. Methods The chromatographic separation was carried out on Kromasil C18 column (250 mm×4.6 mm, 5 μm) with the mobile phase consisting of acetonitrile-0.1% formic acid solution with gradient elution at a flow rate of 1.0 mL/min.The column temperature was 30℃. The detection wavelength were set at 238 nm for feretoside, asperulosidic acid and asperuloside, 280 nm for danshensu, salvianolic acid B and tanshinone ⅡA, and 254 nm for calycosin 7-O-β-D-glucopyranoside and formononetin. Results Feretoside, asperulosidic acid, asperuloside, danshensu, salvianolic acid B, tanshinone ⅡA, calycosin 7-O-β-D-glucopyranoside and formononetin showed good linear relationships within the ranges of 1.49-37.25, 0.76-19.00, 1.07-26.75, 0.99-24.75, 12.61-315.25, 2.43-60.75, 0.69-17.25, 1.86-46.50 μg/mL (r ≥ 0.999 1), whose average recoveries were 98.44%, 98.12%, 99.36%, 97.44%, 100.00%, 99.19%, 96.98%, and 98.93% with the RSD values of 0.71%, 1.47%, 0.98%, 1.35%, 0.85%, 1.13%, 1.02%, and 1.29%, respectively. Conclusion The method is easy to operate and repeatable, can be used for quality control of multiple indicator components in Yiganning Granules."/> A;毛蕊異黃酮葡萄糖苷;芒柄花素;高效液相色譜;Yiganning Granules;feretoside;asperulosidic acid;asperuloside;danshensu;salvianolic acid B;tanshinone ⅡA;calycosin 7-O-β-D-glucopyranoside;formononetin;HPLC"/>

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首頁 > 過刊瀏覽>2020年第35卷第8期 >2020,35(8):1527-1531. DOI:10.7501/j.issn.1674-5515.2020.08.005
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HPLC法測定乙肝寧顆粒中雞矢藤次苷甲酯、車葉草苷酸、車葉草苷、丹參素、丹酚酸B、丹參酮ⅡA、毛蕊異黃酮葡萄糖苷和芒柄花素

Determination of feretoside, asperulosidic acid, asperuloside, danshensu, salvianolic acid B, tanshinone ⅡA, calycosin 7-O-β-D-glucopyranoside, and formononetin in Yiganning Granules by HPLC

發(fā)布日期：2020-08-24
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    [關(guān)鍵詞]
    [摘要]
    目的 建立HPLC法同時測定乙肝寧顆粒中雞矢藤次苷甲酯、車葉草苷酸、車葉草苷、丹參素、丹酚酸B、丹參酮ⅡA、毛蕊異黃酮葡萄糖苷和芒柄花素。方法 采用Kromasil C18色譜柱（250 mm×4.6 mm，5μm）；流動相：乙腈-0.1%甲酸溶液，梯度洗脫；檢測波長分別為238 nm（0~21.0 min檢測雞矢藤次苷甲酯、車葉草苷酸和車葉草苷）、280 nm（21.0~40.0 min檢測丹參素、丹酚酸B和丹參酮ⅡA）和254 nm（40.0~55.0 min檢測毛蕊異黃酮葡萄糖苷和芒柄花素）；體積流量：1.0 mL/min；柱溫：30℃；進(jìn)樣量為10 μL。結(jié)果 雞矢藤次苷甲酯、車葉草苷酸、車葉草苷、丹參素、丹酚酸B、丹參酮ⅡA、毛蕊異黃酮葡萄糖苷和芒柄花素分別在1.49~37.25、0.76~19.00、1.07~26.75、0.99~24.75、12.61~315.25、2.43~60.75、0.69~17.25、1.86~46.50μg/mL線性關(guān)系良好（r ≥ 0.999 1）；各成分平均加樣回收率分別為98.44%、98.12%、99.36%、97.44%、100.00%、99.19%、96.98%、98.93%，RSD值分別為0.71%、1.47%、0.98%、1.35%、0.85%、1.13%、1.02%、1.29%。結(jié)論 該方法操作簡便、重復(fù)性好，可用于乙肝寧顆粒中多指標(biāo)性成分的質(zhì)量控制。
    [Key word]
    [Abstract]
    Objective To establish an HPLC method for simultaneous determination of feretoside, asperulosidic acid, asperuloside, danshensu, salvianolic acid B, tanshinone ⅡA, calycosin 7-O-β-D-glucopyranoside and formononetin in Yiganning Granules. Methods The chromatographic separation was carried out on Kromasil C18 column (250 mm×4.6 mm, 5 μm) with the mobile phase consisting of acetonitrile-0.1% formic acid solution with gradient elution at a flow rate of 1.0 mL/min.The column temperature was 30℃. The detection wavelength were set at 238 nm for feretoside, asperulosidic acid and asperuloside, 280 nm for danshensu, salvianolic acid B and tanshinone ⅡA, and 254 nm for calycosin 7-O-β-D-glucopyranoside and formononetin. Results Feretoside, asperulosidic acid, asperuloside, danshensu, salvianolic acid B, tanshinone ⅡA, calycosin 7-O-β-D-glucopyranoside and formononetin showed good linear relationships within the ranges of 1.49-37.25, 0.76-19.00, 1.07-26.75, 0.99-24.75, 12.61-315.25, 2.43-60.75, 0.69-17.25, 1.86-46.50 μg/mL (r ≥ 0.999 1), whose average recoveries were 98.44%, 98.12%, 99.36%, 97.44%, 100.00%, 99.19%, 96.98%, and 98.93% with the RSD values of 0.71%, 1.47%, 0.98%, 1.35%, 0.85%, 1.13%, 1.02%, and 1.29%, respectively. Conclusion The method is easy to operate and repeatable, can be used for quality control of multiple indicator components in Yiganning Granules.
    [中圖分類號]
    R284.02
    [基金項目]
    天津市自然科學(xué)基金資助項目（18JCYBJC95100）