目的 研究替諾昔康與小牛胸腺DNA（ctDNA）之間的相互作用。方法 借助紫外光譜和熒光光譜考察了替諾昔康與ctDNA之間的相互作用，同時(shí)通過(guò)熱變性研究、黏度法試驗(yàn)考察了替諾昔康與ctDNA的相互作用模式。結(jié)果 ctDNA加入使替諾昔康紫外光譜出現(xiàn)輕微減色效應(yīng)，且隨著ctDNA濃度的增大，減色效應(yīng)變強(qiáng)；替諾昔康的加入使ctDNA-鹽酸小檗堿發(fā)生熒光猝滅，且其猝滅程度具有濃度相關(guān)性。替諾昔康對(duì)于ctDNA溶液的黏度和熱變性溫度影響不大。結(jié)論 替諾昔康與ctDNA是以溝槽方式結(jié)合，并且替諾昔康可以使ctDNA-鹽酸小檗堿發(fā)生靜態(tài)熒光猝滅。

Objective To study the interaction between tenoxicam (TNX) and calf thymus DNA (ctDNA). Methods The interaction mechanism and mode of TNX with ctDNA were investigated by UV-vis absorption and fluorescence spectroscopy, and DNA thermal denaturation and viscosimetric methods were also adopted. Results After adding ctDNA, the UV-vis spectroscopy of TNX showed a slight hypochromicity, which was further intensified with the increase of ctDNA concentration. As the same time, TNX could quench the fluorescence of ctDNA-berberine hydrochloride and its effect was concentration dependent. However, the relative viscosity and melting of ctDNA didn't show significant change with the adding of TNX solution. Conclusion The binding between TNX and ctDNA is groove mode, and TNX quenches the fluorescence of ctDNA-BR by static quenching.

R969.2