目的 研究山藥Dioscorea opposita提取物聯(lián)合樹突細胞-細胞因子誘導(dǎo)的殺傷細胞（DC-CIK）對荷MDA-MB-231乳腺癌干細胞瘤裸鼠的治療效果。方法 制備荷MDA-MB-231乳腺癌干細胞Balb/c裸鼠模型，隨機分為4組，每組8只：對照組裸鼠尾iv生理鹽水，0.2 mL/次，2次/周；DC-CIK組裸鼠在腫瘤干細胞接種4 d后尾iv 1×10⁶個DC-CIK細胞，2次/周，給藥3周；山藥提取物組裸鼠ig山藥提取物125 mg/kg，0.2 mL/d，給藥3周；山藥提取物聯(lián)合DC-CIK組裸鼠ig山藥提取物125 mg/kg，0.2 mL/d，同時尾iv 1×10⁶個DC-CIK細胞，2次/周，給藥3周。各組裸鼠在治療3周期間每2天測量瘤體大小及裸鼠體質(zhì)量，治療結(jié)束后處死裸鼠，取出瘤體稱質(zhì)量；qRT-PCR法檢測瘤組織中Akt信號通路中關(guān)鍵原癌基因c-Myc表達水平。結(jié)果 治療結(jié)束后，各組裸鼠瘤體生長速率為山藥提取物聯(lián)合DC-CIK組結(jié)論 對荷MDA-MB-231乳腺癌干細胞瘤裸鼠治療效果中，各給藥組裸鼠腫瘤生長均受到明顯抑制，其中以山藥提取物聯(lián)合DC-CIK組效果最佳。

Objective To study the therapeutic effect of Dioscoreae Rhizoma extract combined with dendritic cell-cytokine-induced killer cells (DC-CIK) on nude mice bearing MDA-MB-231 breast cancer stem cell tumor. Methods A Balb/c nude mouse model bearing MDA-MB-231 breast cancer stem cells were prepared and randomly divided into 4 groups, 8 in each group: in control group, nude mouse were administrated with normal saline by tail iv, 0.2 mL/time, 2 times/week; Nude mice in DC-CIK group were administrated with 1×10⁶ DC-CIK cells by tail iv after 4 days of tumor stem cell inoculation, twice a week, for 3 weeks; nude mice in Dioscoreae Rhizoma extract group were administrated with Dioscoreae Rhizoma extract 125 mg/kg by ig, 0.2 mL/d, for 3 weeks; nude mice ig Dioscoreae Rhizoma extract + DC-CIK group were administrated with Dioscoreae Rhizoma extract 125 mg/kg by ig, simultaneously were administrated with 1×10⁶ DC-CIK cells by tail iv, twice a week, administration 3 weeks. The tumor volumn and weight of nude mice in each group were measured every 2 days during treatment. After the treatment, the nude mice were sacrificed, and the tumors were taken out and weighed; qRT-PCR method was used to detect expression level of gene c-Myc, the key protocarcinoma in the Akt signaling pathway in tumor tissues. Results After the treatment, the tumor growth rate of nude mice in each group were as follows: Dioscoreae Rhizoma extract + DC-CIK group < DC-CIK group and Dioscoreae Rhizoma extract group < control group. The nude mice in Dioscoreae Rhizoma extract + DC-CIK group, DC-CIK group and Dioscoreae Rhizoma extract + DC-CIK group always had stable mood, normal eating and drinking water, and free movement; nude mice in control group were lethargic, weakened in activity, and eating and drinking decreased. At 3 weeks, the activity was blocked and the tumor was ruptured. Compared with the control group, the c-Myc gene in the tumor of nude mice in each administration group was down-regulated. Conclusion In the treatment effect of nude mice bearing MDA-MB-231 breast cancer stem cell tumor, the tumor growth of nude mice in each administration group was significantly inhibited, and the Dioscoreae Rhizoma extract + DC-CIK group had the best effect.

R285.5