目的 建立黃芪水煎液中黃酮類(lèi)成分HPLC-MS鑒定方法。方法 黃芪水煎液采用C₁₈固相萃取小柱獲得黃酮類(lèi)化學(xué)成分。采用HPLC-DAD-MS的方法對(duì)黃芪水煎液中黃酮類(lèi)成分進(jìn)行了定性研究，對(duì)比所建立化合物庫(kù)的化學(xué)成分與實(shí)測(cè)的化學(xué)成分質(zhì)荷比信息以及化合物的裂解規(guī)律確定各化學(xué)成分。結(jié)果 液相色譜質(zhì)譜聯(lián)用技術(shù)可對(duì)黃芪水煎液所含黃酮類(lèi)成分進(jìn)行快速鑒別。根據(jù)相對(duì)分子質(zhì)量信息確定了黃芪水煎液所含22個(gè)黃酮類(lèi)化學(xué)成分的結(jié)構(gòu)信息。結(jié)論 高效液相-質(zhì)譜聯(lián)用技術(shù)可快速對(duì)黃芪水煎液所含黃酮類(lèi)成分進(jìn)行表征，為其藥效物質(zhì)基礎(chǔ)研究提供新思路。

Objective To establish an identification method for flavonoid chemical constituents in Astragali Radix decoction by HPLC-MS. Methods The samples were prepared by SPE C₁₈ column. HPLC-DAD-MS method was used to identify the flavonoids in Astragali Radix decoction. The chemical constituents of the library were compared with the measured mass charge ratio of chemical composition in Astragali Radix decoction, and the chemical constituents was determined by the pyrolysis law of the compounds. Results HPLC-MS provided high analysis efficiency of chemical components in Astragali Radix decoction. Based on the mass information and MS fragmentation behaviors of the chemical components, 22 compounds in Astragali Radix decoction were identified. Conclusion HPLC-MS technology can quickly characterize the flavonoids in in Astragali Radix decoction, and provide a new idea for the study of its pharmacodynamic material basis

R284.1

天津市自然科學(xué)基金資助項(xiàng)目（18JCYBJC95100）