18色譜柱(250 mm×4.6 mm,5 μm);流動相:乙腈-0.1%磷酸水溶液,梯度洗脫;檢測波長:240 nm(0~27 min檢測龍膽苦苷、馬錢苷酸、獐牙菜苦苷、獐牙菜苷和杯莧甾酮)和208 nm(27~55 min檢測澤瀉醇F、澤瀉醇A、24-乙酰澤瀉醇A、23-乙酰澤瀉醇B);體積流量:1.0 mL/min;柱溫:30℃;進樣量:10 μL。結果 龍膽苦苷、馬錢苷酸、獐牙菜苦苷、獐牙菜苷、杯莧甾酮、澤瀉醇F、澤瀉醇A、24-乙酰澤瀉醇A、23-乙酰澤瀉醇B分別在45.84~1 146.00、9.02~225.50、12.86~321.50、4.69~117.25、0.88~22.00、0.59~14.75、0.77~19.25、0.66~16.50、3.37~84.25 μg/mL與峰面積線性關系良好(r ≥ 0.999 1),平均加樣回收率分別為100.03%、99.55%、99.78%、98.56%、97.98%、96.95%、98.21%、97.75%、100.06%,RSD值分別為0.61%、0.95%、0.71%、1.17%、1.63%、1.09%、1.73%、1.20%、0.84%。結論 所建立的方法結果準確,操作便捷,重復性好,為全面評價痛風定膠囊的整體質量提供了參考依據(jù)。;Objective To establish an HPLC method for simultaneous determination of gentiopicrin, loganic acid, swertiamain, sweroside, cyasterone, alisol F, alisol A, 24-acetate alisol A, and 23-acetate alisol B in Tongfengding Capsules. Methods The separation by HPLC was performed on Waters Symmetry C18 column (250 mm×4.6 mm, 5 μm) with mobile phase of acetonitrile-0.1% phosphoric acid in gradient elution. The detection wavelength were set at 240 nm (for gentiopicrin, loganic acid, swertiamain, sweroside, and cyasterone) and 208 nm (for alisol F, alisol A, 24-acetate alisol A, and 23-acetate alisol B). The flow rate was 1.0 mL/min, the column temperature was 30℃, and the volume of injection was 10 μL. Results Gentiopicrin, loganic acid, swertiamain, sweroside, cyasterone, alisol F,alisol A, 24-acetate alisol A, and 23-acetate alisol B had good linear relationships in the ranges of 45.84-1 146.00, 9.02-225.50, 12.86-321.50, 4.69-117.25, 0.88-22.00, 0.59-14.75, 0.77-19.25, 0.66-16.50, and 3.37-84.25 μg/mL (r ≥ 0.999 1). The average recoveries were 100.03%, 99.55%,99.78%, 98.56%,97.98%, 96.95%, 98.21%, 97.75%, and 100.06% with the RSD values of 0.61%, 0.95%, 0.71%, 1.17%, 1.63%, 1.09%, 1.73%, 1.20%, and 0.84%. Conclusion The established method is accurate, convenient, and reproducible, which provides a reference for the comprehensive evaluation of the overall quality of Tongfengding Capsules."/>