目的 對不同來源的湖南產(chǎn)蓮子中黃曲霉毒素G₁、G₂、B₂、B₁進行高效液相色譜-光化學衍生法測定。方法 采用高效液相色譜-光化學衍生法，采用島津GL Inertsil ODS-3色譜柱（250 mm×4.6 mm，5 μm），流動相為甲醇-乙腈-水（35：13：52），柱溫35 ℃；光化學衍生器（254 nm）激發(fā)波長λ_ex=360 nm，發(fā)射波長λ_ex=450 nm。結(jié)果 黃曲霉毒素G₁、G₂、B₂、B₁分別在6.7～33.3、11.4～56.8、10.3～51.5、4.1～20.3 pg線性關(guān)系良好；平均回收率分別為98.07%、97.72%、96.11%、99.52%，RSD值分別為1.69%、1.40%、2.72%、1.34%（n=6）。9批蓮子樣品中，有6批未檢出黃曲霉毒素，來自于農(nóng)貿(mào)市場農(nóng)戶自存的2批次檢出黃曲霉毒素B₁，實驗室塑料袋包裝儲存1年的1批檢出黃曲霉毒素B₁、B₂，但質(zhì)量分數(shù)均低于法定標準限量。結(jié)論 不同來源湖南產(chǎn)蓮子中黃曲霉毒素質(zhì)量分數(shù)均低于《中國藥典>2020年版規(guī)定限量。該法測定結(jié)果準確、重復(fù)性良好，可為完善蓮子安全性控制和質(zhì)量標準提升提供實驗依據(jù)。

Objective To establish an HPLC-photochemical derivatization method for determination of aflatoxin G₁, G₂, B₂, and B₁ in Nelumbinis Semen from different sources in Hunan Province. Methods The content of aflatoxins were determined by HPLC-photochemical derivatization method. Shimadzu GL Inertsil ODS-3 chromatographic column (250 mm×4.6 mm,5 μm) was used with column temperature of 35℃, and methanol-acetonitrile-water (35:13:52) was used. Fluorescence detector:excitation wavelength λ_ex=360 nm and emission wavelength λ_em=450 nm. Results Aflatoxin G₂, G₁, B₂, and B₁ showed good linear relationships in the ranges of 6.7-33.3, 11.4-56.8, 10.3-51.5, and 4.1-20.3 pg, and their average recoveries were 98.07%, 97.72%, 96.11%, and 99.52% with the RSD values were 1.69%, 1.40%, 2.72%, and 1.34%, respectively. Among 9 batches of Nelumbinis Semen samples, aflatoxin was not detected in 6 batches, and aflatoxin B₁ was detected in 2 batches from farmers' market, and aflatoxin B₁ and B₂ were detected in 1 batch of sample from laboratory plastic bags storage for one year. But the aflatoxin content was much lower than the Chinese Pharmacopoeia 2020 Edition legal standard limit. Conclusion Aflatoxin in Nelumbinis Semen samples of different sources in Hunan Province was lower than the limit stipulated in the Chinese Pharmacopoeia 2020 Edition. The method is accurate and reproducible, which can provide experimental basis for improving the safety control and quality standard of Nelumbinis Semen.

R284.1

湖南省自然科學基金資助項目（2020JJ7066）；湘潭市2020年指導性科技計劃項目（CG-ZDJH2020014）