目的 分析2015—2019年連云港市第二人民醫(yī)院真菌培養(yǎng)陽性標(biāo)本的病原菌分布情況和耐藥狀況。方法 收集2015年1月—2019年12月在連云港市第二人民醫(yī)院所有的真菌培養(yǎng)和藥敏試驗(yàn)陽性的標(biāo)本共663例，應(yīng)用DL-96Ⅱ細(xì)菌測定系統(tǒng)的體外診斷試劑DL-96FOUNGUS對樣本進(jìn)行真菌鑒定，以5種常見抗真菌藥物為例，采用MIC法開展對樣本中已鑒定真菌的藥物敏感性試驗(yàn)，并應(yīng)用WHONET 5.6軟件進(jìn)行對于該試驗(yàn)結(jié)果的統(tǒng)計(jì)學(xué)分析。結(jié)果 2015—2019年連云港市第二人民醫(yī)院共分離出真菌663株。按照菌種類型分類，可得到如下比例：白色假絲酵母菌占49.9%（331/663）、熱帶假絲酵母菌占15.2%（101/663）、都柏林假絲酵母菌占8.3%（55/663）、光滑假絲酵母菌14.9%（99/663）、其他假絲酵母菌占11.6%（77/663）。白色假絲酵母菌對伏立康唑、氟康唑、伊曲康唑3種抗菌藥物仍具有耐藥性；熱帶假絲酵母菌對氟胞嘧啶、兩性霉素高度敏感，對伏立康唑、氟康唑、伊曲康唑表現(xiàn)為耐藥；光滑假絲酵母菌對氟胞嘧啶和兩性霉素B的敏感性最高。結(jié)論 白色假絲酵母菌、熱帶假絲酵母菌、光滑假絲酵母菌是真菌感染的主要常見致病菌，以熱帶假絲酵母菌為主的多種假絲酵母菌對于氟康唑、伊曲康唑等臨床常用抗菌藥物耐藥性日漸增長。

Objective The distribution of pathogens and drug resistance of positive fungal culture specimens in the Second People's Hospital of Lianyungang from 2015 to 2019 were analyzed. Methods A total of 663 fungal culture and drug sensitivity test positive specimens were collected from The Second People's Hospital of Lianyungang from January 2015 to December 2019. The in vitro diagnostic reagent DL-96FOUNGUS of DL-96Ⅱ bacterial determination system was used to identify the fungi in the samples. Taking 5 common antifungal drugs as examples, the MIC method was used to carry out drug sensitivity test of identified fungi in the samples, and WHONET 5.6 software was used for statistical analysis of the test results. Result According to the types of bacteria, the following proportion can be obtained:Candida albicans accounted for 49.9% (331/663), Candida tropicalis accounted for 15.2% (101/663), Candida Dublin accounted for 8.3% (55/663), Candida glabrata accounted for 14.9% (99/663), and other candida accounted for 11.6% (77/663). Candida albicans was still resistant to voriconazole, fluconazole, and itraconazole. Candida tropicali was highly sensitive to fluorocytosine and amphotericin, and showed resistance to voriconazole, fluconazole and itraconazole. Candida glabrata had the highest sensitivity to fluorocytosine and amphotericin B. Conclusion Candida albicans, Candida tropicalis, and Candida globulidis are the main pathogenic bacteria of fungal infection. The resistance of various candida strains, mainly the Candida tropicalis, to fluconazole, itraconazole, and other commonly used antibiotics in clinic is increasing day by day.

R969.3

江蘇大學(xué)臨床醫(yī)學(xué)科技發(fā)展基金項(xiàng)目（JLY2021088）