目的 研究神經(jīng)生長因子（NGF）對缺血缺氧性腦損傷大鼠學習記憶能力的影響。方法 選取30只21 d齡的雄性SD大鼠，隨機分為假手術(shù)組、模型組和NGF干預組，利用左側(cè)頸動脈結(jié)扎結(jié)合低氧環(huán)境建立缺血缺氧性腦損傷模型，以大鼠出現(xiàn)左旋、翻身和衡異常則為造模成功。NGF干預組于造模成功后次日通過大鼠立體定位儀icv NGF溶液，40 μg/kg，2次/周，共處理4周，模型組和假手術(shù)組給予等量生理鹽水注射。采用BBB評分、懸吊試驗、斜坡試驗評價大鼠四肢運動能力，水迷宮實驗評價大鼠定位航行和空間探索實驗學習記憶能力，并取腦組織皮質(zhì)區(qū)進行HE染色觀察其病理變化。結(jié)果 NGF干預后大鼠的BBB評分、懸吊試驗時間提高，而斜坡試驗時間縮短，差異有統(tǒng)計學意義（P<0.05）；NGF干預后大鼠找到平臺時間和定位航行中潛伏期、游泳距離縮短，在第I象限和中環(huán)停留的時間延長，且差異具有統(tǒng)計學意義（P<0.05）。HE染色結(jié)果顯示，與假手術(shù)組相比，模型組大鼠皮質(zhì)區(qū)神經(jīng)細胞排列紊亂且數(shù)目明顯減少，并可見炎性細胞浸潤，部分出現(xiàn)變性和壞死，而NGF干預后神經(jīng)細胞排列紊亂及神經(jīng)細胞數(shù)目減少明顯改善，變性及壞死細胞數(shù)目也明顯較少。結(jié)論 NGF可能是通過改善缺血缺氧性腦損傷模型大鼠神經(jīng)功能損傷，使神經(jīng)組織功能恢復，從而提高了神經(jīng)運動及學習記憶能力。

Objective To study the effects of nerve growth factor (NGF) on learning and memory abilities of rats with hypoxic-ischemic brain damage. Methods Thirty male SD rats aged 21 d old were enrolled and randomly divided into sham operation group, model group, and NGF intervention group. Hypoxic-ischemic brain damage models were established by left carotid artery ligation combined with hypoxic environment. If there was levorotation, turning over and abnormal balance, the modeling was successful. NGF intervention group was injected with NGF solution 40 μg/kg twice a week in the lateral ventricle for 4 weeks on the next day after the successful modeling. Model group and sham operation group were injected with normal saline. BBB score, suspension test, and slope test were applied to evaluate motor ability of rats. Water maze test was applied to evaluate learning and memory ability in place navigation and space exploration experiments. The cortical area of brain tissue was taken to observe pathological changes by HE staining. Results After NGF intervention, BBB score and time of suspension test were increased, while time of slope test was shortened (P < 0.05). After NGF intervention, time of rats finding out platform, latency and swimming distance in place navigation were shortened, staying time in the first quadrant and the middle ring was prolonged (P < 0.05). The results of HE staining showed that compared with sham operation group, arrangement of nerve cells in cortical area was disordered, and their number was significantly reduced in model group. There was inflammatory cell infiltration, and some were accompanied with degeneration and necrosis. After NGF intervention, disordered arrangement of nerve cells and decreased nerve cells number were significantly improved, and number of degeneration and necrosis cells was also significantly less. Conclusion NGF may recover nerve tissue function by improving nerve function damage in cerebral palsy rats, thus improve nerve motor, learning and memory ability.

R971

河南省衛(wèi)生計生科技項目（2018077）