目的 采用注射野百合堿（MCT）制備大鼠肺動(dòng)脈高壓模型，并對(duì)肺動(dòng)脈壓測(cè)定方法進(jìn)行優(yōu)化。方法 通過(guò)ip MCT誘導(dǎo)建立大鼠肺動(dòng)脈高壓模型，通過(guò)測(cè)定大鼠肺血管阻力的方法來(lái)對(duì)大鼠右心室壓進(jìn)行測(cè)定，采用陽(yáng)性藥對(duì)照、血流動(dòng)力學(xué)測(cè)定和組織學(xué)觀察確定模型是否建立成功。結(jié)果 ip 50 mg/kg MCT 4周后大鼠右心室平均壓、右心肥大指數(shù)對(duì)比對(duì)照組明顯增高，觀察病理組織切片，可見(jiàn)明顯的內(nèi)皮細(xì)胞損傷，分布不均，動(dòng)脈管壁明顯增厚，肺組織有大量的炎性細(xì)胞浸潤(rùn)，出現(xiàn)肺血管的重構(gòu)，證實(shí)肺動(dòng)脈高壓動(dòng)物模型造模成功。結(jié)論 采用ip MCT可以建立穩(wěn)定的大鼠肺動(dòng)脈高壓模型，且具有較高的存活率，同時(shí)建議采用改良的心導(dǎo)管測(cè)定方法測(cè)定大鼠右心室壓力。

Objective To establish pulmonary hypertension model of rats by injection of monocrotaline (MCT), and to optimize measurement method of pulmonary arterial pressure. Methods Rat pulmonary hypertension model was established by ip MCT induction, and right ventricular pressure was measured by measuring pulmonary vascular resistance in rats. Positive drug control, hemodynamic measurement, and histological observation were used to determine whether the model was established successfully. Results Four weeks after ip MCT rat average pressure, right heart right ventricular hypertrophy index were increased compared to control group obviously, observed the pathological tissue slice, visible obvious endothelial cell damage, uneven distribution, the artery wall thickening obviously, there are a large number of inflammatory cells infiltration, lung tissue of pulmonary vascular reconstruction, confirmed that the pulmonary hypertension animal model building success. Conclusion Intraperitoneal injection of 50 mg/kg MCT can establish a stable rat model of pulmonary hypertension with a high survival rate, and it is recommended to use an improved cardiac catheterization method to measure right ventricular pressure in rats.

R965

云南省高層次衛(wèi)生計(jì)生技術(shù)人才培養(yǎng)經(jīng)費(fèi)資助（H-2019026）；云南省科技廳-昆明醫(yī)科大學(xué)應(yīng)用基礎(chǔ)研究聯(lián)合專項(xiàng)資金項(xiàng)目[2018FE001（-082）]