目的 探究附子瀉心湯治療慢性萎縮性胃炎的作用機(jī)制。方法 利用TCMSP數(shù)據(jù)庫(kù)篩選附子瀉心湯4味中藥的活性成分及相應(yīng)靶點(diǎn)；利用DisGeNet數(shù)據(jù)庫(kù)篩選慢性萎縮性胃炎相關(guān)靶點(diǎn)，運(yùn)用Cytoscape軟件構(gòu)建疾病–藥物–靶點(diǎn)網(wǎng)絡(luò)；通過(guò)STRING數(shù)據(jù)庫(kù)進(jìn)行蛋白互作網(wǎng)絡(luò)（PPI）分析，得到核心靶點(diǎn)。利用R語(yǔ)言“clusterprofiler”包進(jìn)行基因功能GO分析和KEGG通路富集分析。結(jié)果 共得到附子瀉心湯87個(gè)活性成分，216個(gè)作用靶點(diǎn)，得到慢性萎縮性胃炎相關(guān)靶點(diǎn)639個(gè)，藥物與疾病交集靶點(diǎn)85個(gè)，其中核心靶點(diǎn)包括AKT1、TP53、VEGF、TNF、PTGS2、IL6、JUN、CASP3等。附子瀉心湯主要參與脂多糖應(yīng)答、對(duì)細(xì)菌起源的分子應(yīng)答、活性氧化物代謝、氧化應(yīng)激、金屬離子應(yīng)答、信號(hào)通路調(diào)節(jié)等生物過(guò)程，通過(guò)調(diào)節(jié)PI3K-Akt信號(hào)通路、AGE-RAGE信號(hào)通路、乙型肝炎等通路發(fā)揮治療慢性萎縮性胃炎的作用。結(jié)論 附子瀉心湯可通過(guò)多靶點(diǎn)、多機(jī)制、多途徑治療慢性萎縮性胃炎。

Objective To explore the mechanism of Fuzi Xiexin Decoction in treating chronic atrophic gastritis. Methods The active components and target sites of 4 herbs of Fuzi Xiexin Decoction were screened by TCMSP database, the target sites of chronic atrophic gastritis were screened by DisGeNet database, and the disease-drug-target network was constructed by Cytoscape software PPI analysis was carried out by STRING database to get the core target, and GO analysis and KEGG pathway enrichment analysis were carried out by "clusterprofiler" package. Results There were 87 active components, 216 active targets, 639 chronic atrophic gastritis related targets, and 85 drug-disease intersection targets, the key targets included AKT1, TP53, VEGF, TNF, PTGS2, IL-6, Jun, and CASP3. Fuzi Xiexin Decoction is mainly involved in the biological processes of lipopolysaccharide response, molecular response to bacterial origin, active oxide metabolism, oxidative stress, metal ion response, signal pathway regulation, etc. PI3K-Akt signaling pathway, AGE-RAGE signaling pathway, hepatitis B pathway play a role in treatment of chronic atrophic gastritis. Conclusion Fuzi Xiexin Decoction can treat chronic atrophic gastritis through multiple targets, multiple mechanisms and multiple pathways.

R285

江蘇省第五期"333工程"培養(yǎng)資金資助項(xiàng)目[蘇中醫(yī)科教（2018）4號(hào)]