目的 對(duì)比胃、腸溶膠囊遞送鼠李糖乳桿菌的效果。方法 通過(guò)單次ig給予鼠李糖乳桿菌胃溶膠囊和腸溶膠囊，觀察鼠李糖乳桿菌在大鼠腸道中不同腸段處的分布，比較胃溶膠囊和腸溶膠囊遞送鼠李糖乳桿菌至大鼠腸道的情況。給藥后通過(guò)鼠李糖乳桿菌特異性引物進(jìn)行RT-PCR檢測(cè)乳桿菌濃度。結(jié)果 相比于空膠囊組，胃溶膠囊和腸溶膠囊組大鼠的小腸和結(jié)腸腸段內(nèi)容物中鼠李糖乳桿菌的濃度均顯著升高。腸溶膠囊組比胃溶膠囊組更能顯著地提高小腸中鼠李糖乳桿菌濃度，腸溶膠囊組進(jìn)入結(jié)腸需要的時(shí)間比胃溶膠囊組長(zhǎng)，但后期其進(jìn)入結(jié)腸后，也能顯著提高結(jié)腸中鼠李糖乳桿菌濃度。結(jié)論 與胃溶膠囊相比，腸溶膠囊能夠更好地保護(hù)鼠李糖乳桿菌到達(dá)腸道，且使用RT-PCR測(cè)定腸道中鼠李糖乳桿菌濃度的方法特異性強(qiáng)，實(shí)驗(yàn)結(jié)果較為可靠，可為臨床應(yīng)用提供有用的參考。

Objective The distribution of lactobacillus rhamnosus in different intestinal segments of rats was observed by single intragastric administration of lactobacillus rhamnosus in gastric capsule and enteric capsule, and the delivery of lactobacillus rhamnosus in intestinal tracts of rats was compared. Methods The distribution of Lactobacillus rhamnosus in different intestinal segments of rats was observed by single gavage of Lactobacillus rhamnosus gastric and enteric-soluble capsules, and the delivery of Lactobacillus rhamnosus to the intestine of rats was compared. After administration, lactobacillus concentration was detected by real-time PCR using specific primers of Lactobacillus rhamnosus. Results Compared with the empty capsule group, the concentrations of Lactobacillus rhamnosus in the small intestine and colon of rats in gastric capsule and enteric-capsule groups were significantly increased. The enteric-capsule group could significantly increase the concentration of Lactobacillus rhamnosus in the small intestine than the stomach-capsule group, and the time of enteric-capsule group to enter the colon was longer than that of stomach-capsule group, but the enteric-capsule group could also significantly increase the concentration of Lactobacillus rhamnosus in the colon after its late entry into the colon. Conclusion Compared with stomach dissolved capsules, enteric dissolved capsules can better protect Lactobacillus rhamnosus to reach the intestinal tract, and real-time PCR method for the determination of Lactobacillus rhamnosus concentration is highly specific, the experimental results are more reliable, which can provide a useful reference for clinical application.

R944