目的 優(yōu)選芪苓益腎顆粒的提取工藝。方法 以黃芪甲苷得率、總多糖得率、干膏得率的綜合評分為評價指標(biāo)，采用層次分析法（AHP）確定這3個指標(biāo)權(quán)重系數(shù)，設(shè)計L₉（3⁴）正交試驗篩選最佳加水量、提取次數(shù)和提取時間，并進(jìn)行驗證試驗。結(jié)果 以AHP法確定的權(quán)重系數(shù)合理有效。篩選的最優(yōu)提取工藝為加8倍量水，提取3次，每次1 h。3次驗證試驗結(jié)果顯示，黃芪甲苷、總多糖、干膏得率的平均值分別為0.10%、2.51%、43.09%；平均綜合評分為99.88，RSD值為0.03%。結(jié)論 正交試驗設(shè)計結(jié)合AHP確定的多指標(biāo)權(quán)重系數(shù)法可較好地優(yōu)化芪苓益腎顆粒的提取工藝。

Objective To optimize the extraction technology of Qiling Yishen Granules. Methods The comprehensive scores of astragaloside IV yield, total polysaccharides yield, and dry extract yield were used as the evaluation index, and the weight coefficient of these indexes was determined by analytic hierarchy process (AHP). L₉ (3⁴) orthogonal test was designed to screen water volume, extraction times, and extraction time, and validation tests were carried out. Results The weight coefficient determined by AHP method was reasonable and effective. The optimized extraction technology was to add 8-fold water, extracting 3 times, and 1 h each time. The results of three validation tests showed that the average yields of astragaloside IV, total polysaccharides, and dry paste were 0.10%, 2.51%, and 43.09%, respectively. Average comprehensive score was 99.88 with RSD value 0.03%. Conclusion Orthogonal test and multi-index weighting analysis method could be used to optimized extraction process of Qiling Yishen Granules.

R284.2

河南省醫(yī)學(xué)科技攻關(guān)計劃項目（LHGJ20191429）