目的 基于網(wǎng)絡(luò)藥理學(xué)方法探討生姜治療類風(fēng)濕關(guān)節(jié)炎（RA）的可能分子作用機制。方法 通過TCMSP平臺篩選生姜主要活性成分，利用UniProt數(shù)據(jù)庫將相應(yīng)靶點蛋白名稱進(jìn)行轉(zhuǎn)化為基因名，經(jīng)Cytoscape 3.8.2軟件構(gòu)建網(wǎng)絡(luò)圖；在GeneCards數(shù)據(jù)庫、OMIM數(shù)據(jù)庫、TTD數(shù)據(jù)庫中檢索“rheumatoid arthritis”，獲取RA疾病靶點，制作韋恩圖獲取生姜藥物成分和RA共同靶點，并進(jìn)行蛋白質(zhì)相互作用（PPI）網(wǎng)絡(luò)分析、基因本體論（GO）功能富集及京都基因與基因組百科全書（KEGG）通路分析。利用分子對接技術(shù)預(yù)測有效成分與靶點的潛在結(jié)合活性。結(jié)果 獲取生姜5個成分及55個潛在靶點，RA疾病4 836個相關(guān)靶點，生姜與RA共同靶點26個，PPI網(wǎng)絡(luò)中自由度靠前的3個靶點基因為前列素內(nèi)環(huán)氧化物合成酶2（PTGS2）、轉(zhuǎn)錄因子AP-1（JUN）、半胱氨酸天冬氨酸蛋白酶-3（CASP3），GO功能富集分析和KEGG通路分析分別得到185個結(jié)果和59條信號通路。結(jié)論 生姜治療RA具有多成分、多靶點效應(yīng)，并涉及多條信號通路，這可能通過PTGS2、JUN、CASP3等靶點調(diào)控炎癥反應(yīng)、免疫調(diào)節(jié)作用治療RA。

Objective To investigate the possible mechanism of Zingiberis Rhizoma Recens in treatment of rheumatoid arthritis(RA)by network pharmacology. Methods The activeingredients of Zingiberis Rhizoma Recens were screened by TCMSP platform, UniProt database was used to convert the corresponding target protein name into gene name, and the network map was constructed by Cytoscape 3.8.2 software. "rheumatoid arthritis" was searched in GeneCards, OMIM, and TTD databases to obtain RA disease targets. Venn diagram was made to obtain the common targets of Zingiberis Rhizoma Recens drug components and RA. PPI network analysis, GO enrichment and KEGG pathway enrichment analysis were performed. The potential binding activity of active ingredient and target was predicted by molecular docking technique. Results Five components and 55 potential targets of Zingiberis Rhizoma Recens were obtained, including 4 836 related targets of RA disease and 26 common targets of Zingiberis Rhizoma Recens and RA. The three target genes with higher degree of freedom in PPI network were prost endocyclic oxide synthase 2 (PTGS2), transcription factor AP-1 (JUN) and cysteine aspartate protease-3 (CASP3). GO functional enrichment analysis and KEGG pathway analysis yielded 185 results and 59 signaling pathways, respectively. Conclusion Zingiberis Rhizoma Recens treatment of RA has multi-component, multi-target effect, involving multiple signaling pathways, and may regulate inflammatory response and immunomodulation through PTGS2, JUN, CASP3 and other targets to treat RA.

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國家自然科學(xué)基金資助項目（82160869）；貴州省科技計劃項目（黔科合支撐[2021]一般006）；貴州省科技計劃項目（黔科合平臺人才[2020]2202號）；貴州省中醫(yī)藥管理局科學(xué)技術(shù)研究課題（QZYY-2020-021）；貴州省高層次創(chuàng)新型人才項目[（2016）5650]