目的 通過網(wǎng)絡(luò)藥理學(xué)探討黃連溫膽湯治療心律失常的機(jī)制。方法 通過TCMSP平臺(tái)收集黃連溫膽湯相關(guān)藥物的有效成分及作用靶點(diǎn)；經(jīng)過UniProt獲取標(biāo)準(zhǔn)蛋白名并轉(zhuǎn)換靶點(diǎn)基因名；使用DIGSEE、OMIN、Drugbank等數(shù)據(jù)庫收集心律失常疾病基因靶點(diǎn)，合并后去重與復(fù)方中藥靶點(diǎn)取交集；使用String數(shù)據(jù)庫繪制蛋白相互作用（PPI）網(wǎng)絡(luò)，使用Cytoscape 3.9.1經(jīng)過拓?fù)浞治霁@取核心靶點(diǎn)；使用R語言進(jìn)行京都基因與基因組百科全書（KEGG）及基因本體（GO）富集分析并對(duì)排名靠前的通路進(jìn)行可視化處理；對(duì)KEGG排名靠前的通路使用Cytoscape 3.9.1構(gòu)建中藥成分–靶點(diǎn)–通路圖。結(jié)果 通過拓?fù)浞治龅玫奖砥どL因子受體（EGFR）、絲裂原活化蛋白激酶（MAPK）1、MAPK3、信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子3（STAT3）、CAMP反應(yīng)性元素結(jié)合蛋白1（CREB1）、雌激素受體（ESR1）等19個(gè)核心靶點(diǎn)；通過KEGG富集分析共得到186條信號(hào)通路，黃連溫膽湯可能通過晚期搪基化終產(chǎn)物及其受體（AGE-RAGE）、磷脂酰肌醇3-激酶（phosphatidylinositol-3-kinase，PI3K）/蛋白激酶B（protein kinase B，Akt）、MAPK等信號(hào)通路對(duì)心律失常起治療作用。結(jié)論 黃連溫膽湯可通過多種目標(biāo)基因、分子通路治療心律失常。

Objective To explore the mechanism of Huanglian Wendan Decoction in treatment of arrhythmia based on network pharmacology. Methods The effective components and targets of Huanglian Wendan Decoction were collected by TCMSP platform. The standard protein name was obtained by UniProt and the target gene name was converted. The gene targets of arrhythmia were collected by DIGSEE, OMIN, Drugbank, and other databases. PPI protein interaction network was drawn using String database, and the core target was obtained using Cytoscape 3.9.1 through topological analysis. KEGG and GO enrichment analysis was performed using R language, and the top-ranked pathways were visualized. Cytoscape 3.9.1 was used to construct TCM component-target-pathway map for pathways with high KEGG ranking. Results 19 Core targets including EGFR, MAPK1, MAPK3, STAT3, CREB1, and ESR1 were obtained. A total of 186 signaling pathways were obtained through KEGG enrichment analysis. Huanglian Wendan Decoction may have therapeutic effects on arrhythmia through AGE-RAGE, PI3K-Akt, and MAPK and other signaling pathways. Conclusion Huanglian Wendan Decoction can treat arrhythmia through multiple target gene and molecular pathways.

R972

江西省自然科學(xué)基金資助項(xiàng)目(20202BABL206141);國家自然科學(xué)基金項(xiàng)目(81960820);全國中醫(yī)藥創(chuàng)新骨干人才[國中醫(yī)藥人教函(2019)128號(hào)];江西省中醫(yī)藥中青年骨干人才[贛中醫(yī)藥科教字[2020]2號(hào)]