目的 研究紫草素對(duì)慢性鼻竇炎小鼠鼻黏膜組織炎癥反應(yīng)及沉默信息調(diào)控因子1（SIRT1）/核因子E2相關(guān)因子2（Nrf2）信號(hào)通路的影響。方法 選取80只小鼠建立慢性鼻竇炎模型后隨機(jī)分為模型組，紫草素12.5、50.0 mg/kg組，克拉霉素組，每組20只。另取正常飼養(yǎng)的20只小鼠設(shè)為假手術(shù)組。各組小鼠分別ig給予相應(yīng)藥物，1次/d，共14 d。采用蘇木素-伊紅（HE）染色觀察小鼠鼻黏膜組織病理形態(tài)學(xué)；Masson染色觀察小鼠鼻黏膜組織膠原沉積情況；ELISA法檢測(cè)小鼠血清中炎性因子水平；Western blotting實(shí)驗(yàn)檢測(cè)小鼠鼻黏膜組織中SIRT1/Nrf2信號(hào)通路相關(guān)蛋白表達(dá)。結(jié)果 與模型組比較，紫草素12.5、50.0 mg/kg組小鼠鼻竇黏膜慢性炎癥表現(xiàn)和藍(lán)色膠原沉積明顯改善；血清中腫瘤壞死因子-α（TNF-α）、白細(xì)胞介素-32（IL-32）、γ干擾素（IFN-γ）水平顯著降低；鼻黏膜組織中SIRT1、Nrf2、血紅素氧合酶-1（HO-1）、NADPH醌氧化還原酶1（NQO-1）蛋白表達(dá)水平顯著升高（P＜0.05）。且紫草素50.0 mg/kg組小鼠上述指標(biāo)均顯著優(yōu)于紫草素12.5 mg/kg組（P＜0.05）。結(jié)論 紫草素能夠改善慢性鼻竇炎小鼠鼻黏膜組織重塑和炎癥反應(yīng)，其作用機(jī)制可能與激活SIRT1/Nrf2信號(hào)通路有關(guān)。

Objective To study the effects of shikonin on the inflammatory response of nasal mucosa and silent information regulator 1 (SIRT1)/ nuclear factor E2-related factor 2 (Nrf2) signaling pathway in mice with chronic rhinosinusitis. Methods Eighty mice were selected to establish chronic sinusitis model and were randomly divided into model group, shiksin 12.5, 50.0 mg/kg group, and clarithromycin group, with 20 mice in each group. Another 20 mice in normal feeding were selected as sham operation group. Each group was given the corresponding drug intragastrically, once daily, for 14 days. The pathological morphology of mice nasal mucosa was observed by HE staining, collagen deposition in mice nasal mucosa was observed by Masson staining, the level of inflammatory factors in mice serum were detected by ELISA method, and the expression of SIRT1/Nrf2 signaling pathway related proteins in mice nasal mucosa were detected by Western blotting. Results Compared with model group group, the chronic inflammation and blue collagen deposition of nasal sinuses mucosa in shikonin 12.5 and 50.0 mg/kg group were significantly improved, the levels of TNF-α, IL-32, and IFN-γ in serum were decreased, and the expression levels of SIRT1, Nrf2, HO-1, and NQO-1 protein in nasal mucosa were increased (P < 0.05). The above indexes of mice in shikonin 50.0 mg/kg group were significantly better than those in shikonin 12.5 mg/kg group (P < 0.05). Conclusion Shikonin can improve the tissue remodeling and inflammatory response of nasal mucosa in chronic rhinosinusitis, and its mechanism may be related to the activation of SIRT1/Nrf2 signaling pathway.

R285.5

四川省衛(wèi)生健康科研課題資助項(xiàng)目（19PJ067）