目的 以“腸-關(guān)節(jié)”軸為切入點，探討金藤清痹顆粒抗類風(fēng)濕關(guān)節(jié)炎的作用機制。方法 按體質(zhì)量將大鼠隨機分為6組，即對照組、模型組、雙氯芬酸鈉組以及金藤清痹顆粒高、中、低劑量組，每組6只。采用膠原誘導(dǎo)法進行造模，設(shè)計金藤清痹顆粒高、中、低劑量組給藥劑量分別為6.30、3.15、1.58 g/kg；雙氯芬酸鈉組給藥劑量為3 mg/kg；對照組和模型組ig等量生理鹽水，給藥4周。蘇木精-伊紅染色法（HE）觀察大鼠踝關(guān)節(jié)部位病理變化，酶聯(lián)免疫吸附測定法（ELISA）檢測大鼠血清相關(guān)炎癥指標，16S rDNA分析盲腸內(nèi)容物中腸道菌群變化。結(jié)果 與模型組比較，金藤清痹顆?？擅黠@降低膠原誘導(dǎo)型關(guān)節(jié)炎模型大鼠關(guān)節(jié)腫脹度，減少踝關(guān)節(jié)炎性細胞浸潤，同時可顯著降低膠原誘導(dǎo)型關(guān)節(jié)炎大鼠血清腫瘤壞死因子-α（TNF-α）、白細胞介素（IL）-1β、C反應(yīng)蛋白（CRP）水平（P＜0.05、0.01）；16S rDNA結(jié)果顯示，金藤清痹顆粒6.30 g/kg可增加Cyanobacteria、軟壁菌門、疣微菌門、梭桿菌門、毛螺菌科NK4A136群和Ruminiclostridium 5等菌群的相對豐度，以及降低變形菌門、乳桿菌屬和瘤胃球菌屬1等菌群的相對豐度。結(jié)論 金藤清痹顆?？赡芡ㄟ^調(diào)控“腸-關(guān)節(jié)”軸發(fā)揮抗類風(fēng)濕關(guān)節(jié)炎的作用。

Objective To tnvestigated the mechanism of Jinteng Qingbi Granules against rheumatoid arthritis based on gut microbiota. Methods According to body weight, the rats were randomly divided into 6 groups, namely control group, model group, diclofenac sodium group, and Jinteng Qingbi Granules high, medium, and low-dose groups, with 6 rats in each group. Collagen induction method was used for modeling, and the dosages of Jinteng Qingbi Granules in high, medium, and low dose groups were 6.30, 3.15,1.58 g/kg, respectively. The dose of diclofenac sodium group was 3 mg/kg; Control group and model group were given the same amount of normal saline intragastrically for 4 weeks. Hematoxylin-eosin staining (HE) was used to observe the pathological changes of the ankle joint of the rats, ELISA was used to detect the serum inflammatory indicators, and 16S rDNA was used to analyze the changes of intestinal flora in the cecum contents. Results Compared with the model group, Jinteng Qingbi Granules could significantly reduce the degree of joint swelling, the infiltration of ankle arthritic cells, and the serum levels of TNF-α, IL-1β and CRP in collagen-induced arthritis model rats. At the same time, 16S rDNA showed that Jinteng Qingbi Granules could increase the relative abundance of Cyanobacteria, Tenericutes, Verrucomicrobia, Fusobacteria, Lachnospiraceae NK4A136 group, and Ruminiclostridium 5, as well as decrease the relative abundance of Proteobacteria, Lactobacillus and Ruminococcus 1. Conclusion Jinteng Qingbi Granules may play an anti-rheumatoid arthritis role by regulating the “gut-joint” axis.

R979.5

河南省醫(yī)學(xué)科技攻關(guān)計劃聯(lián)合共建項目（LHGJ20190275）