目的 建立UPLC-MS/MS法同時(shí)測(cè)定香砂平胃顆粒中蒼術(shù)素、厚樸酚、和厚樸酚、橙皮苷、蕓香柚皮苷和甘草苷。方法 色譜條件采用Waters HSS T3 C₁₈色譜柱（100 mm×2.1 mm，1.7 μm），流動(dòng)相為乙腈-0.1%甲酸水，梯度洗脫，體積流量0.3 mL/min，柱溫40 ℃，進(jìn)樣量5 μL。質(zhì)譜條件選擇離子源：電噴霧離子源（ESI），采集模式：多反應(yīng)檢測(cè)（MRM），正負(fù)離子同時(shí)掃描，離子源溫度150 ℃，毛細(xì)管電壓：正離子3.0 kV，負(fù)離子2.5 kV，去溶劑氣流量800 L/h，去溶劑氣溫度400 ℃，氬氣為高純氬氣。結(jié)果 甘草苷、蕓香柚皮苷、橙皮苷、和厚樸酚、厚樸酚、蒼術(shù)素線性范圍在0.29～14.75、0.38～18.58、1.85～92.63、1.62～81.11、1.37～68.5、0.49～24.41 ng，平均回收率分別為100.48%、97.79%、98.67%、93.52%、101.72%、100.30%，RSD值分別為4.31%、3.40%、1.45%、2.78%、2.59%、4.06%。結(jié)論 本法分析速度快、靈敏度高、專(zhuān)屬性強(qiáng)，可以為香砂平胃顆粒的質(zhì)量標(biāo)準(zhǔn)改進(jìn)提供依據(jù)。

Objective To develop an UPLC-MS/MS method for the simultaneous determination of atractylodin, honokiol, magnolol, hesperidin, naringin, and liquiritin in Xiangsha Pingwei Granules. Methods The chromatographic condition was Waters HSS T3 C₁₈ column (100 mm × 2.1 mm, 1.7 μm), mobile phase acetonitrile -0.1% formic acid solution with gradient elution, column temperature set at 40 ℃, and injection volume of 5 μL with flow rate of 0.3 mL/min. The mass spectrum condition with ESI ions source, acquisition mode: MRM, simultaneous scanning of positive and negative ions, ion source temperature 150 ℃, capillary voltage: positive ions 3.0 kV, negative ions 2.5 kV, desolvent gas flow 800 L/h, desolvent gas temperature 400 ℃, and argon as high-purity argon. Results Liquiritin, naringin, hesperidin, magnolol, honokiol, and atractylodin showed good linear relationships in the concentration range of 0.29-14.75, 0.38-18.58, 1.85-92.63, 1.62-81.11, 1.37-68.5, and 0.49-24.41 ng. The average recoveries were 100.48%, 97.79%, 98.67%, 93.52%, 101.72%, and 100.30% with RSD values of 4.31%, 3.40%, 1.45%, 2.78%, 2.59%, and 4.06%, respectively. Conclusion The method is fast, sensitive, and specific, and can provide a basis for improving the quality standard of Xiangsha Pingwei Granules.

R286.02