目的 采用高效液相色譜-四級桿/靜電場軌道阱高分辨質(zhì)譜（HPLC-Q-Exactive-Orbitrap-MS）法鑒定森登-4味涂劑中化學(xué)成分。方法 色譜條件：Shim-pack GIST-HP C₁₈色譜柱（150 mm×4.6 mm，5 μm），流動相為乙腈-0.1%甲酸水溶液，梯度洗脫，體積流量0.35 mL/min，柱溫為40 ℃，進(jìn)樣量為5 μL。質(zhì)譜條件：檢測方式Full MS/dd-MS²，掃描范圍m/z 110～1 200。電噴霧離子源（ESI），正、負(fù)離子模式掃描，噴霧電壓4.20 kV（+）、3.80 kV（-），碰撞能量（CE）30 eV。通過全方化學(xué)成分信息庫，結(jié)合多級質(zhì)譜碎片信息、對照品保留時間，鑒定森登-4味涂劑中化學(xué)成分。結(jié)果 在森登-4味涂劑中共鑒定出60個成分，包括16個黃酮類成分、13個有機(jī)酸類成分、11個環(huán)烯醚萜類成分、10個鞣質(zhì)類成分、4個氨基酸類成分、4個脂肪酸類成分、2個三萜類成分，并考察了山梔苷、槲皮素、阿魏酸、克里拉京、齊墩果酸、脯氨酸、亞油酸的MS²圖和可能的裂解途徑。結(jié)論 對森登-4味涂劑中化學(xué)成分進(jìn)行了快速、準(zhǔn)確的鑒定，為其質(zhì)量標(biāo)準(zhǔn)建立、藥效物質(zhì)挖掘和作用機(jī)制研究提供了實驗基礎(chǔ)。

Objective To identify the chemical components in Sendeng-4 Pigmentum by HPLC-Q-Exactive-Orbitrap-MS. Methods Chromatographic conditions: Shim-pack GIST-HP C₁₈ column (150 mm × 4.6 mm, 5 μm) was employed with the mobile phase of acetonitrile - 0.1% formic acid solution for gradient elution. The flow rate was 0.35 mL/min, the column temperature was set at 40 ℃, and the volume of sample injection was 5 μL. Mass spectrometry conditions: Detection method: Full MS/dd MS², scanning range m/z 110 — 1 200. Electrospray ionization (ESI) was applied for scanning positive and negative ion mode scanning with spray voltage 4.20 kV (+) and 3.80 kV (-), and collision energy was 30 eV. Comprehensive chemical composition information database, combined with multi-level mass spectrometry fragment information and reference substance retention time were used to identify the chemical components in Sendeng-4 Pigmentum. Results A total of 60 components were identified in Sendeng-4 Pigmentum, including 16 flavonoids, 13 organic acids, 11 iridoids, 10 tannins, 4 amino acids, 4 fatty acids, and 2 triterpenes. MS² and possible fragmentation pathway of shanzhiside, quercetin, ferulic acid, corilagin, oleanolic acid, proline, and linoleic acid were investigated. Conclusion Chemical components in Sendeng-4 Pigmentum were quickly and accurately identified, providing an experimental basis for the establishment of its quality standards, the exploration of pharmacological substances, and its mechanism of action.

R286.02

內(nèi)蒙古自治區(qū)自然科學(xué)基金資助項目（2022LHQN08001）；內(nèi)蒙古醫(yī)科大學(xué)聯(lián)合項目（YKD2022LH031）；內(nèi)蒙古醫(yī)科大學(xué)實驗室開放基金項目（2022ZN15，2022ZN16）；內(nèi)蒙古醫(yī)科大學(xué)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計劃項目（202210132052）