目的 建立華山參的UPLC指紋圖譜，鑒別其正品和混偽品。方法 建立華山參的UPLC指紋圖譜，對相似度進行分析，采用聚類分析、主成分分析、偏最小二乘-判別分析法對共有峰進行分析。結(jié)果 建立的指紋圖譜共標定出15個共有峰。華山參正品的相似度均在0.867以上，混偽品的相似度均低于0.555。30批華山參樣品聚為3類，正品與偽品、主產(chǎn)地與非主產(chǎn)地華山參藥材被顯著區(qū)分，并篩選出4個差異性成分。結(jié)論 建立的UPLC指紋圖譜能有效鑒別華山參及其混偽品，為華山參藥材的質(zhì)量評價和真?zhèn)舞b別提供參考。

Objective To establish an UPLC fingerprint of Physochlainae Radix to identify Physochlainae Radix and its adulterants. Methods UPLC fingerprint of Physochlainae Radix was established, the similarity was analyzed. The common peaks were analyzed by cluster analysis, principal component analysis, and partial least squares-discriminant analysis. Results A total of 15 characteristic peaks were calibrated in the established fingerprint. Similarities of Physochlainae Radix were all above 0.867, and the similarity of adulterants were all less than 0.555. Thirty batches of Physochlainae Radix samples were clustered into 3 categories. Superior and inferior products of Physochlainae Radix, as well as primary and non-primary producing areas, were significantly distinguished, and 4 quality difference markers were screened. Conclusion The established UPLC fingerprint can effectively identify Physochlainae Radix and its adulterants, providing reference for quality evaluation and identification of Physochlainae Radix medicinal materials.

R286.02

現(xiàn)代中醫(yī)藥海河實驗室科技項目（22HHZYJC00005）