目的 探討美登木素對(duì)人乳頭狀甲狀腺癌B-CPAP細(xì)胞增殖、凋亡的影響及作用機(jī)制。方法 用不同濃度（0.19、0.76、3.05、12.21、48.83、195.31、781.25、3 125 nmol/L）的美登木素溶液處理B-CPAP細(xì)胞，SRB法檢測B-CPAP細(xì)胞增殖情況。將B-CPAP細(xì)胞分為對(duì)照組及美登木素（0.1、1、10 nmol/L）組，光學(xué)顯微鏡觀察B-CPAP細(xì)胞形態(tài)變化；流式細(xì)胞術(shù)檢測B-CPAP細(xì)胞凋亡情況；Western blotting法測定B-CPAP細(xì)胞中B淋巴細(xì)胞瘤-2（Bcl-2）、半胱氨酸天冬氨酸蛋白酶-3前體（Procaspase-3）的蛋白表達(dá)量。結(jié)果 與對(duì)照組比較，美登木素能有效抑制B-CPAP細(xì)胞增殖，且呈濃度及時(shí)間相關(guān)性；隨美登木素濃度增加B-CPAP細(xì)胞凋亡率逐漸升高，Bcl-2和Procaspase-3蛋白表達(dá)水平下降（P＜0.05），呈明確的濃度相關(guān)性。結(jié)論 美登木素具有抑制B-CPAP細(xì)胞增殖及誘導(dǎo)凋亡的作用，其機(jī)制可能與調(diào)控Bcl-2和Procaspase-3蛋白有關(guān)。

Objective To investigate the effects of maytansine on the proliferation and apoptosis of B-CPAP cells and its mechanism. Methods B-CPAP cells were treated with different concentrations (0.19, 0.76, 3.05, 12.21, 48.83, 195.31, 781.25, and 3 125 nmol/L) of maytansine solution. The proliferation of B-CPAP cells was detected by SRB. B-CPAP cells were divided into control group and maytansine (0.1, 1, and 10 nmol/L) group. The change of cellular morphology was observed by invert microscope, and the flow cytometry was used to detect the cell apoptosis rate and the expression of Bcl-2 and Procaspase-3 protein was assayed by Western blotting. Results Compared with the control group, maytansine could proliferation inhibited B-CPAP cell in concentration and time-dependent manner. The apoptosis rate of B-CPAP cells gradually increased, and the expression levels of Bcl-2 and Procaspase-3 proteins decreased (P < 0.05) with concentration dependent. Conclusion Maytansine could inhibit the proliferation and induce apoptosis of B-CPAP cells, and its mechanism may be related to the regulation of Bcl-2 and Procaspase-3 proteins.

R285.5

滄州市重點(diǎn)研發(fā)計(jì)劃項(xiàng)目（204106038）；天津市第二批衛(wèi)生健康行業(yè)高層次人才選拔培養(yǎng)工程（TJSJMYXYC-D2-034）；天津市衛(wèi)生健康委天津市中醫(yī)藥管理局中醫(yī)中西醫(yī)結(jié)合科研課題（2021072）