目的 建立茜草多指標(biāo)成分定量聯(lián)合化學(xué)計(jì)量學(xué)評(píng)價(jià)不同產(chǎn)地茜草的質(zhì)量。方法 采用高效液相色譜法測(cè)定8省區(qū)18批茜草中新橙皮苷、山柰酚、茜草素、異茜草素、羥基茜草素、1,3,6-三羥基-2-甲基蒽醌、大葉茜草素、去氫-α-拉杷醌、β-谷甾醇和異落葉松脂素，并采用主成分分析、正交偏最小二乘法-判別分析、灰色關(guān)聯(lián)度分析對(duì)測(cè)定結(jié)果進(jìn)行評(píng)價(jià)。結(jié)果 相同產(chǎn)區(qū)的茜草，質(zhì)量較接近；共找到4個(gè)差異性標(biāo)志成分，其影響顯著性排序?yàn)榇笕~茜草素＞茜草素＞1,3,6-三羥基-2-甲基蒽醌＞羥基茜草素；陜西、河南和安徽所產(chǎn)的茜草整體質(zhì)量較好。結(jié)論 方法便捷、準(zhǔn)確，易于推廣，可以更全面、更系統(tǒng)地評(píng)價(jià)茜草的質(zhì)量。

Objective To establish a method for quality evaluation of Rubiae Radix by HPLC combined with chemometrics. Methods The contents of neohesperidin, kaempferol, alizarin, xanthopurpurin, purpurin, 1,3,6-trihydroxy-2-methylanthraquinone, mollugin, dehydro-α-lapachone, β-sitosterol, and isolariciresinol in 18 batches of Rubiae Radix collected from 8 provinces were determined by HPLC method, and the results were analyzed by principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), and grey relational analysis (GRA) method. Results Rubiae Radix from the same production area had similar quality. A total of four differential marker components were found, and their significance was ranked as follows: mollugin ＞ alizarin ＞1,3,6-trihydroxy-2-methylanthraquinone ＞ purpurin. The overall quality of Rubiae Radix in Shaanxi, Henan, and Anhui Province was good. Conclusion The method is convenient, accurate, and easy to promote, which can comprehensively and systematically evaluate the quality of Rubiae Radix.

R286.02

河南省醫(yī)學(xué)科技攻關(guān)計(jì)劃省部共建指導(dǎo)性計(jì)劃重點(diǎn)項(xiàng)目（SBGJ202002105）